新生SD大鼠G418耳蜗Corti器离体损伤模型的建立  

Cochlea Corti organ insult model by G418 in newborn SD rats in vitro

作　　者：吴保俊[1] 祝康[1] 韦俊荣[1] 郑国玺[1] 杨广笑[2] 王全颖[2] 

机构地区：[1]西安交通大学医学院第二附属医院耳鼻喉科,西安710004 [2]西安华广生物工程有限公司

出　　处：《陕西医学杂志》2008年第10期1275-1277,1326,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(30471877)

摘　　要：目的:建立毒性蛋白G418损坏新生大鼠耳蜗毛细胞的离体培养试验模型。方法:应用耳蜗基底膜分离取材技术,耳蜗器官离体培养技术和毛细胞免疫组化染色技术定量观察了G418对新生SD小鼠耳蜗毛细胞的损害。结果:G418对耳蜗毛细胞的有明显的损害,200μg/ml的终浓度,内外毛细胞均有不同程度损害,以外毛细胞为著。结论:G418可用于建立耳蜗Corti器离体损伤模型。Objective:To establish a G418 damage model to the hair cells of newborn rats incochlear organotypic cultures. Methods:Cochlear microdissection, cochlear organotypic cultures, and hair cell Immunohistochemical staining were used to quantify the ototoxic effects of G418. Results:G418 destroys cochlear hair cells obviously at a dose of 200 μg/ml. The inner and outer hair cells damaged at different degree, severely in outer hair cells. Conclusion:G418 could be usedto establish cochlear organotypic damage model ...

关 键 词：耳蜗 CORTI器 @G418 

分 类 号：R764[医药卫生—耳鼻咽喉科]