SLE患者外周血单个核细胞IFN-γ及IL-10mRNA表达与疾病活动性的相关性  

Relationship between the expression of IFN-γ and IL-10 mRNA and disease activity in patients with systemic lupus erythematosus

作　　者：卢宝彦[1] 王子娥[1] 刘义庆[1] 李枫[1] 邵松[1] 卢志明[1] 

机构地区：[1]山东大学附属省立医院,山东济南250021

出　　处：《山东医药》2008年第20期1-2,共2页Shandong Medical Journal

基　　金：山东省自然科学基金资助项目(Y2006C116)。

摘　　要：目的探讨系统性红斑狼疮(SLE)患者疾病活动性的相关因素。方法选择70例SLE患者(SLE组)和63例健康查体者(对照组),采用PCR法检测外周血单个核细胞IFN-γmRNA和IL-10 mRNA表达情况;采用凝胶图像扫描系统对PCR产物的电泳条带进行密度扫描。结果SLE组IL-10 mRNA表达量高于对照组(P<0.01),IFN-γ表达低于对照组,但无统计学差异。SLE组SLE疾病活动指数(SLEDAI)>10分者IFN-γmRNA表达显著高于≤10分者,IL-10 mRNA表达在两者间无统计学差异。结论SLE时存在Th1细胞向Th2细胞漂移现象;IFN-γ表达增高与SLE疾病活动性密切相关。Objective To analyze the correlation factor of disease activities in patients with systemic lupus erythematosus(SLE).Methods The expression of IFN-γ and IL-10 mRNA were amplified by using semi-quantitative reverse transcriptase-polymerase chain reaction(RT-PCR).The density of electrophoresis band in PCR products was scanned by gel scanning system.Results The expression of IL-10 mRNA in PBMCs of patients with SLE was significantly higher than that of the healthy control group(P<0.01),the expression of IFN...

关 键 词：红斑狼疮 系统性 TH1细胞 TH2细胞 γ-干挠素 白细胞介素-10 

分 类 号：R593.241[医药卫生—内科学]