Comparison of Molecular Biological Techniques (RAPD, ARDRA and SSrRNA Gene Sequencing) on the Phylogenetic Relationships of Lower Taxa within Spirotrichous Ciliates  

作　　者：陈子桂 SONG Weibo 

机构地区：[1]Laboratory of Protozoology, MKL, Ocean University of Qingdao, Qingdao, 266003,P.R.China

出　　处：《High Technology Letters》2002年第4期1-6,共6页高技术通讯（英文版）

基　　金：SupportedbytheNaturalScienceFoundationofChina (No .3 9970 0 98) ;theCheungKongScholarshipProgrammeandaresearchgrantbytheEducationalMinistryofChina (No .990 77)

摘　　要：The evolutionary relations of seven spirotrichous ciliates (3 stichotrichs: oxytricha saltans, oxytricha ferruginea, stylonychia mytilus; 4 hypotrichs: Uronychia transfuga , diophrys appendiculata, aspidisca steini, euplotes vannus) inferred from the SSrRNA (small subunit rRNA) gene sequences and the polymorphic patterns of RAPD (random amplified polymorphic DNA) and ARDRA (amplified ribosomal DNA restriction analyses) fingerprinting are constructed. Compared with that of morphometric characters, the dendrograms from SSrRNA gene using three calculation methods (distance matrix, maximum-parsimony, and UPGMA) agree with the morphological division into two clades, oxytricha - stylonychia and uronychia - diophrys - aspidisca - euplotes, though the branching orders within the hypotrichous ciliates are slightly different from morphometric analyses.The evolutionary relationships of seven spirotrichous ciliates (3 stichotrichs: Oxytricha saltans, O. Ferruginea, Stylonychia mytilus; 4 hypotrichs: Uronychia transfuga, Diophrys appendiculata, Aspidisca steini, Euplotes vannus ) inferred from the SSrRNA (small subunit rRNA) gene sequences and the polymorphic patterns of RAPD (random amplified polymorphic DNA) and ARDRA (amplified ribosomal DNA restriction analyses) fingerprinting are constructed. Compared with that of morphometric characters, the dendrograms from SSrRNA gene using three different calculation methods (distance matrix, maximum parsimony, and UPGMA) agree with the morphological division into two clades, Oxytricha Stylonychia and Uronychia Diophrys Aspidisca Euplotes, though the branching orders within the hypotrichous ciliates are slightly different from morphometric analyses.

关 键 词：phylogeny polymorphic DNA RAPD ARDRA SSrRNA gene 

分 类 号：Q78[生物学—分子生物学]