鸡传染性法氏囊病毒3个毒株VP2高变区的基因扩增与序列分析  

The amplifing and sequence analyses of the highly variable VP2 region of 3 IBDV strains

作　　者：张艳英[1] 孙继国[1] 郑世学[1] 孙玉杰 张曼夫[2] 

机构地区：[1]河北农业大学动物科技学院,河北保定071001 [2]中国农业大学生物学院,北京100094

出　　处：《河北农业大学学报》2002年第z1期240-242,共3页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金委资助项目 (3 0 0 115 )

摘　　要：以用疫苗株V -hb和V1免疫后又发病的病鸡法氏囊分离毒株HeB -bdI的基因组RNA为模板 ,利用RT -PCR/Nested -PCR扩增出了cDNA产物。并对其VP2高变区基因序列测定 ,同时测定了V -hb和V1的基因序列。序列分析表明 :HeB -bdI毒株与UK661同源性较高为 98.4% ,而与弱毒株Cu -1、非致病性株PBG98和变异株相差较大 ;而疫苗株V -hb和V1疫苗株与HeB -bdI毒株的同源性较低仅为 93 .1%。one single DNA fragments was obtained from strains HeB bdI that isolated from the diseased chickens who were vaccined by 2 vaccine strains V1 and V hb Then VP2 cDNA was directly sequenced by sanger's DNA sequencing method and then the amino acid sequences were deduced. Both the nucleotide sequence and amino acid sequences were compared with five published sequence of highly variable VP2 region of IBDV serotype I strains. It was shown that HeB bdI were mostly closely related to the very virulent strains UK661, but different from the low pathogenic strain CU 1 and non pathogenic strain PBG98. The cloning and sequence analyses of the highly variable VP2 Region of 2 vaccine strains V1 and V-hb were done meanwhile.

关 键 词：传染性法氏囊病毒 超强毒株 VP2 基因克隆 序列分析 

分 类 号：S852.65[农业科学—基础兽医学]