Observation on human ovarian theca cell and granulosa cell interaction in polycystic ovary syndrome  

作　　者：焦泽旭 周灿权 庄广伦 梁晓燕 

机构地区：[1]Reproductive Medical Center,The First Affiliated Hospi-tal of Sun Yet-Sen University of Medical Sciences,Guangzhou 510080 [2]Reproductive Medicai Center,The First Affiliated Hospi-tal of Sun Yet-Sen University of Medical Sciences,Guangzhou 510080

出　　处：《生殖医学杂志》2002年第z1期60-64,共5页Journal of Reproductive Medicine

摘　　要：Objective: To investigate the role of human theca cell(TC) and granulo sa cell(GC) interaction and insulin(INS) in steroidogenesis in normal ovarian cy cle and in patients with PCOS. Methods: Ovarian theca and granulosa cells from eleven normal wo men and eight PCOS patients were co-cultured on opposite side of collagen with or without INS. The concentrations of estradiol(E2), progesterone(P) and andro stenedione (A) in the culture medium were examined by ELISA method.Results: When co-cultured with GC, TC in PCOS group produced mo re A and less P than those of normal group. When co-cultured with theca cells, granulosa cells in PCOS group produced more E2 than those of normal group. Add ition of INS increased the difference significantly.Conclusions: The GC and TC interaction from the normal and PCOS ovaries is different. There is a high A and high E2 intraovary loop of PC OS leading to premature arrest of follicle growth and anovulation. Insulin may p lay an important regulatory role.Objective:To investigate the role of human theca eell(TC) and granulosa cell (GC) interaction and insulin(INS) in steroidogenesis in normal ovarian cycle and in patients with PCOS. Methods:Ovarian theca and granulosa cells from eleven normal women and eight PCOS patients were co-cultured on opposite side of collagen with or without INS.The concentrations of estradiol (E_2),progesterone (P) and androstenedione (A) in the cul- ture medium were examined by ELISA method. Results:When co-cultured with GC,TC in PCOS group produced more A and less P than those of normal group.When co-cultured with theca cells,granulosa cells in PCOS group produced more E_2 than those of normal group.Addition of INS increased the difference significantly. Conclusions:The GC and TC interaction from the normal and PCOS ovaries is dif- ferent.There is a high A and high E_2 intraovary loop of PCOS leading to premature ar- rest of follicle growth and anovulation.Insulin may play an important regulatory role.

关 键 词：POLYCYSTIC OVARY syndrome GRANULOSA cell THECA c ell INSULIN 

分 类 号：R[医药卫生]