移植静脉中基质金属蛋白酶-9的表达对内膜增生的作用  

Effect of Matrix Metalloproteinase-9 on Intimal Hyperplasia of Vein Graft

作　　者：罗涛[1] 杨巍[1] 冯众一[1] 张强[1] 段志泉[1] 

机构地区：[1]中国医科大学第一临床学院外科,辽宁沈阳110001

出　　处：《中国医科大学学报》2002年第z1期19-21,共3页Journal of China Medical University

摘　　要：目的:探讨移植静脉中基质金属蛋白酶-9的变化对内膜增生的作用。方法:建立大鼠颈静脉-腹主动脉自体静脉移植模型,分别在术后3,7,14d进行取材。采用免疫组织化学方法及明胶酶谱方法观察基质金属蛋白酶-9的表达,计算机图像分析仪观测内膜厚度的变化。结果:静脉移植后基质金属蛋白酶-9活性增高,伴有内膜显著增生,二者呈现平行关系。施加基质金属蛋白酶-9抑制剂强力霉素后,内膜受到明显抑制,呈现量效关系。结论:基质金属蛋白酶-9是内膜增生的重要因素之一。强力霉素可以抑制内膜增生。Objective: This study investigated the role of matrix metalloproteinse-9 ( MMP-9 ) in rat model of auto vein graft. Methods: A rat vein graft model was constructed by interposing a jugular vein into the abdominal aorta with end-to-end anastomoses. At the 3rd,7th,and 14th day after the operation, segments of interplanted vein were obtained from Wister rats. Serial sections of vein samples were examined by using Verhoeff elastin staining and MMP-9 immumohisto-chemistry staining. Measurements of neointimal thickness were made using a computerized image analysis system. The quantification of the MMP-9 was tested by substrate gel enzymography. Results: The expression of MMP-9 tented to increase after vein graft. The expression of MMP-9 expression began at the 3rd day, before the formation of intimal hyperpla-sia. Peak expression of MMP-9 occurred by 7 days and remained high, parallelling the development of intimal hyperplasia. The role of MMP-9 in intimal hyperplasis was further investigated by using doxycycline. The intima thickness of rats which received doxycycline was significantly lower than that of rats received nothing ( P <0. 01). We also found that dose-dependent relationship existed between the two groups which received different dosage( P <0. 01). Conclusion: MMP-9 played an important role in imtimal hyperplasia. Doxycycline can significantly reduce neointimal thickening in our model.

关 键 词：基质金属蛋白酶-9 内膜增生 移植静脉 

分 类 号：R541.1[医药卫生—心血管疾病]