基于线扫描方式的激光共焦显微镜的研究  

Study on Line-scanning Based Laser Confocal Microscopy

作　　者：吴开杰[1] 李刚[1] 虞启琏[1] 金霞[1] 

机构地区：[1]天津大学精密测试技术及仪器国家重点实验室,天津300072

出　　处：《仪器仪表学报》2002年第z1期134-135,共2页Chinese Journal of Scientific Instrument

摘　　要：激光共焦扫描显微镜 (L CSM)是一种新型光学显微镜 ,它能够对活体组织的深度结构进行清晰的二维或三维成像。由于传统共焦扫描显微镜采用单点扫描方式 ,成像速度慢 ,无法满足活体组织实时成像的要求。介绍一种以线扫描方式代替点扫描方式的激光共焦扫描显微镜。线扫描方式不但简化了扫描机构的设计 ,而且提高了扫描速度 。Laser confocal scanning microscopy (LCSM) is a new kind of optical microscopy,which can obtain 2D or 3D imaging of depth structure of living tissue with high resolution.Because of traditional LCSM's single point scanning,whose imaging velocity is very low,it can't fulfil real time imaging of living tissue.This paper introduces a new LCSM which substitutes line scanning mode for point scanning mode.Line scanning mode not only makes the design of scanning mechanism simple,but also increases scanning velocity.Thus,it is possible to carry through real time imaging of living tissue.

关 键 词：激光共焦扫描显微镜 柱面镜 线扫描 层析成像 

分 类 号：TP2[自动化与计算机技术—检测技术与自动化装置]