人离体肝组织胺类产生与死亡时间和环境温度的关系  

Study on the relationship between organic amines derived from putrefaction of isolated human hepatic tissues and the time of death under different environmental temperatures

作　　者：张艳苓[1] 邓小军[1] 卢延旭[1] 孙双龙[1] 王庆丰[1] 惠军成 宋春阳 

机构地区：[1]中国刑事警察学院,辽宁沈阳110035 [2]迁安市公安局刑警技术中队 [3]崇礼县公安局刑警大队,河北崇礼076550

出　　处：《中国法医学杂志》2002年第6期338-340,共3页Chinese Journal of Forensic Medicine

摘　　要：目的探讨在不同环境温度下,人离体肝组织中有机胺类产生量随死亡时间变化的规律,为某些碎尸案的死亡时间推断提供参考资料。方法应用高效液相色谱仪外标法,动态检测人离体肝组织中组胺、腐胺、尸胺和未腐败氨基酸含量。结果在8℃、15℃、23℃和32℃环境下,有机胺类产生量分别于人死后肝脏即刻离体69h、54h、40h和33h趋于最大值,并且在到达峰值之前,各温度组离体肝组织中有机胺类生成量均与死亡时间成正比关系。结论人离体肝组织中有机胺类产生量与死亡时间和环境温度在一定范围内均呈正相关。Objective To search some references for estimating the time of death in some murder cases by observation the dynamic changes of organic amines derived from isolated human hepatic tissues. Method The contents of histamines, putrescines, cadaverines and undecomposed amino acids from the isolated human hepatic ussues were assayed dynamically by the method of high performance liquid chromatography (HPLC). Results The contents of the organic amines produced by the putrefaction under 8℃,15℃ , 23℃ and 32℃ were increased along with the time elapsed after death under four environmental temperatures before attaining to their peak value, While the contents of the undecomposed amino acids were decreased gradually after death. Conclusion The amount of organic amines produced by putrefaction of the isolated human hepatic tissues were directly related to the time of death. So do the environmental temperature.

关 键 词：法医病理学 人离体肝组织 有机胺类 死亡时间 HPLC 

分 类 号：D919[医药卫生—法医学]