人胚神经干细胞的体外培养  

Cultivation of Human Embryo Neural Stem Cell in vitro

作　　者：王兰[1] 胡火珍[1] 李小玉[1] 陶大昌[1] 陈芬[1] 

机构地区：[1]四川大学生命科学院细胞生物学教研室,成都610041

出　　处：《四川大学学报（自然科学版）》2002年第S1期204-207,共4页Journal of Sichuan University(Natural Science Edition)

摘　　要：目的　探讨人胚神经干细胞体外培养的条件和分化情况 ,以摸索出一种切实可行的能获得较纯且多潜能人胚神经干细胞的方法 .方法　采用取 3月龄人胎脑 ,用胰蛋白酶消化法分离单个细胞 ,部分冻存 ,另一部分进行细胞培养 ,加EGF ,bFGF刺激生长 ,有限稀释法获得单细胞克隆 ,血清诱导分化 ,并用免疫组化方法进行鉴定 .结果　EGF和bFGF同时存在于无血清培养基中 ,有大量神经干细胞团生成 ,含血清培养基则诱导神经干细胞分化成为神经元、星型胶质细胞、少突胶质细胞 .结论　神经干细胞的存活和分裂有赖于EGF和bFGF的共同作用 .经冻存后的胎脑细胞同样能分离培养出有活性的神经干细胞 .Objective To study the cultivation condition in vitro and differentiation of neural stem cells from the embryonic human in order to find a way to get purified multipotential neural stem cells. Methods The single-cells were derived from the three-month-human embryonic brain digested with trypsin,some cells were frozen, the other cells were expanded with EGF and bFGF, the single -cell-clone were obtained by the way of limited dilution, the serum was used to induce the cells differentiation . The cells wer...

关 键 词：人胚 神经干细胞 细胞培养 细胞冻存 EGF BFGF 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]