二仙汤及其拆方对GT_(1-7)细胞株GnRH基因转录和表达的影响  

Effect of Erxian Decoction and Its Dissected Recipe on Gonadotropin Releasing Hormone Gene Transcription and Expression of GT_(1-7)Cell Line

作　　者：陈名道[1] 杨颖[1] 李凤英[1] 唐金凤[1] 高国锋[1] 殷峻[1] 陈家伦[1] 

机构地区：[1]上海第二医科大学附属瑞金医院上海市内分泌研究所,上海200025

出　　处：《中国中西医结合杂志》2002年第S1期220-222,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助课题(No.39970927)

摘　　要：目的:运用血清药理学方法,观察补肾方二仙汤及其温肾、滋阴两个拆方对 GT_(1-7)细胞 GnRH基因转录和表达的影响。方法:SD 大鼠连续4天灌服中药二仙汤及两个拆方,末次给药1 h 后取其血清作体外细胞培养实验;用放免法测定 GT_(1-7)细胞 GnRH 的分泌;用 Northern bolt 方法观察二仙汤及其拆方对 GT_(1-7)细胞 GnRH mRNA 的影响。结果:(1)二仙汤全方及其两个拆方均能促进 GnRH 释放,以全方效果最好。(2)孵育24 h 后全方组和温肾组 GnRH mRNA 水平有下降趋势。(3)孵育48 h 后滋阴组 GnRH mRNA 的表达明显增加。结论:中医“肾主生殖”的功能似涉及下丘脑 GnRH 神经元及其调控性腺轴的机能,二仙汤及其拆方能够直接调节 GT_(1-7)细胞 GnRH 的释放及基因表达。Objective:To observe the effect of Erxian Decoction(EXD) and its two dissected recipes,the recipe of warming Kidney(WK) and the recipe of norurishing Yin (NY),on gonadotropin releasing hormone (GnRH) gene transcription and expression of CT_(1-7) cell line,using serum-pharmacological method.Methods:SD rats were medicated with EXD,WK and NY for 4 consecutive days,their sera were collected 1h after the last medication for in vitro cell culture.GnRH secretion of GT_(1-7) cells was determined by radioimmunoassay...

关 键 词：GT_(1-7)细胞株 GNRH 二仙汤及其拆方 

分 类 号：R285.5[医药卫生—中药学]