鸡血藤等4种中药对类风湿性关节炎患者单个核细胞IFN-γ、IL-10基因转录的影响  

Effect of Spatholobus Suberectus,Sinomenium Acutum,Siegesbeckia Orientalis and Caesalpinia Sappan on Gene Transcription Level of Interferon-γ,Interleukin-10 in the Peripheral Blood Mononuclear Cells of Rheumatoid Arthritis Patients

作　　者：缪珠雷[1] 熊飚[2] 王韧[1] 

机构地区：[1]上海中医药大学微生物学与免疫学教研室,上海200032 [2]上海市针灸研究所

出　　处：《中国中西医结合杂志》2002年第S1期207-209,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:研究鸡血藤(Sptholobus suberectus Dunn.),青风藤[Sinomenium acutum(Thunb.)Re-hd.et Wils.],风湿草(Siegesbecuia orientalis L.),苏木(Caesalpinia sappan L.)4种中药治疗类风湿性关节炎(RA)的免疫学机理。方法:采用半定量 RT-PCR 方法检测上述4种中药水提取液刺激 RA 患者外周血单个核细胞(PBMC)后,其γ-干扰素(IFN-γ)、白细胞介素10(IL-10)基因转录的变化。结果:添加中药后,RA 患者外周血 PBMC IFN-γ、IL-10基因的转录均有明显下降。结论:上述4种中药对 RA 患者异常活化的细胞免疫和体液免疫有纠正作用,是治疗 RA 有效的机理之一。Objective:To explore the immunoregulation of 4 species of Chinese herbal medicine(CHM),Spatholobus suberectus,Sinnomenium acutum,Siegesbeckia orientalis and Caesalpinia sappan,which were used for curing rheumatoid arthritis(RA),Methods:The gene tran- scription level of interferon-γ(IFN-γ),interleukin-10(IL-10)in the peripheral blood mononuclear cell(PBMC)of RA patients was examined by using semi-quantitative RT-PCR after the PBMCs were stimulated by these CHM.Results:The expression of IFN-γ,IL- 10 was down-...

关 键 词：鸡血藤 青风藤 风湿草 苏木 半定量 RT-PCR IFN-γ IL-10 

分 类 号：R259[医药卫生—中西医结合]