土贝母诱导人胃癌细胞凋亡和分化的研究  

Effect of Rhizoma Bolbostemmae in Inducing Apoptosis and Differentiation of Gastric Carcinoma Cells

作　　者：李蒙[1] 于建勋[2] 孙冬 钱雪松[1] 胡素贤[2] 叶红军[2] 

机构地区：[1]解放军第208医院,长春130062 [2]吉林大学第二临床学院 [3]长春市中医学院

出　　处：《中国中西医结合杂志》2002年第S1期201-203,共4页Chinese Journal of Integrated Traditional and Western Medicine

摘　　要：目的:观察土贝母制剂对 SGC-7901人胃癌细胞的影响。方法:采用 MTT 分析法、流式细胞仪分析技术及透射电镜技术,体外实验观察土贝母诱导人胃癌细胞凋亡和分化。结果:当土贝母制剂浓度在5～200 μg/ml 范围内时,癌细胞抑制率呈浓度依赖性;浓度为20、50、80 μg/ml 时,分别对 SGC-7901细胞进行12 h 的作用,G_0加 G_1期细胞比率(与对照组比较)明显增加(P<0.05),S 期细胞比率明显减少(P<0.05),SGC-7901细胞可发生凋亡;20、50 μg/ml 作用12 h 时,在电镜下观察到典型的细胞凋亡的形态学改变,细胞核的变化最为突出。结论:土贝母制剂能阻止 G_0加 G_1期 SGC-7901细胞向 S 期进程,低浓度时可诱导人胃癌细胞凋亡。Objective:To observe the effect of Bolbostemmae(Bol)preparation on human gastric carcinoma cell line SGC-7901.Methods: Apoptosis and differentiation of SGC-7901 cells were observed in vitro by MTT,flow cytometry and transmission electron microscopy (TEM).Results:Inhibition rate of SGC-7901 cells by Bol was concentration-dependent when the concentration of Bol ranged within 5 -200μg/ml.Using Bol in 20μg/ml,50μg/ml and 80μg/ml acted on SGC-7901 cells for 12hrs could cause obvious increase of G_0/ G_1 ratio,de...

关 键 词：土贝母 胃癌 细胞凋亡 分化 

分 类 号：R285.5[医药卫生—中药学]