海珠益肝胶囊对小鼠免疫性肝损伤肝细胞凋亡的影响  

Effect of Haizhu Yigan Capsule on Liver Cell Apoptosis Induced by Immunized Liver Damage to the Mice

作　　者：盛国光[1] 王东华[1] 李卫星[1] 淡大琦 晏雪生[1] 

机构地区：[1]湖北中医学院附属医院,武汉430061

出　　处：《中国中西医结合杂志》2002年第S1期144-146,共4页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：湖北省自然科学基金课题(No.2000J035)

摘　　要：目的:探讨海珠益肝胶囊对免疫性肝损伤模型肝细胞凋亡及病理变化的影响。方法:雄性昆明种小鼠72只,随机分成正常对照组、模型组、海珠益肝胶囊大、中、小剂量组和地塞米松组。采用卡介苗(BCG)加脂多糖(LPS)作尾静脉注射诱导免疫性肝损伤模型,于造模第12天,分离血清,统一检测丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST),取肝组织进行病理学检测及用原位先端标记法检测肝细胞凋亡。结果:海珠益肝胶囊有如下作用:(1)明显降低小鼠血清 ALT、AST(P<0.01)。(2)明显改善 BCG 加LPS 诱导的小鼠肝病理损伤,其中以大剂量组效果最好。(3)使免疫性肝损伤小鼠肝细胞凋亡减少,并且有剂量依赖关系。结论:海珠益肝胶囊对 BCG 加 LPS 所致免疫性肝损伤,具有保肝降酶、改善肝组织病理损伤和抑制肝细胞凋亡的作用。Objective:To explore the effect of Haizhu Yigan Capsule(HZYGC)on liver cell apoptosis and pathological change in animal model of immune liver injury.Methods:Seventy-two male Kunming mice were randomly divided into the normal control group,the model group,the three HZYGC groups(treated with high,middle and low dose respectively)and the dexamethasone group.The mouse liver in- jury model was induced by injecting BCG and lipopolysaccharide(LPS)through caudal vein.Blood serum of mice was collected on the 12th da...

关 键 词：肝损伤 细胞凋亡 海珠益肝胶囊 

分 类 号：R285.5[医药卫生—中药学]