塞隆骨原动物高原鼢鼠核基因18S rRNA序列测定与分析  

Nuclear Ribosomal RNA Small Subunit (18S rRNA) Nucleotide Sequencing and Characterization of Sailonggu(Whole Bone of Myospalax baileyi Thomas)

作　　者：曹晖 刘玉萍 张绍来 周开亚 

机构地区：[1]中国北京同仁堂集团公司北京同仁堂药酒厂 [2]南京师范大学遗传资源研究所 [3]北京 [4]江苏南京

出　　处：《中国实验方剂学杂志》2002年第S1期-,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市自然科学基金资助课题 (7982 0 2 8)

摘　　要：目的 :测定仓鼠科动物高原鼢鼠Myospalaxbaileyi的核rDNA基因序列 ,为塞隆骨正品基原检定提供分子依据。方法 :采用PCR直接测序技术测定高原鼢鼠 18SrRNA基因核苷酸序列并作序列特征分析。结果 :高原鼢鼠的 18SrRNA序列长度为 185 1bp。根据排序比较 ,高原鼢鼠与 2种鼠科动物间的DNA序列同源性为 72 .0 4 %～ 72 .18%。结论 :通过基因序列分析 。Objective: Sequencing the nuclear ribosomal RNA small subunit (18S rRNA) gene of Myospalax baileyi (Cricetidae) to develop an ultimate and definitive means for origin identification of genuine Sailonggu. Methods: The total DNA was prepared from dried tail tissues. The nuclear 18S rRNA gene region was amplified by PCR using a consensus primer set and its nucleotide sequence was determined by PCR direct sequencing. The characteristic analysis of 18S rRNA sequences was generated usin software program Genetyx-...

关 键 词：塞隆骨 高原鼢鼠 18S RRNA基因 DNA测序 

分 类 号：R29[医药卫生—民族医学]