纯化大鼠胚肝细胞脾内移植增殖的实验研究  

Improvement of proliferation of intrasplenically transplanted fetal hepatocytes after immunoadsorption

作　　者：徐国权[1] 周晓东[2] 魏菁[2] 闵军[1] 陈积圣[1] 

机构地区：[1]中山医科大学孙逸仙纪念医院普外科,广州510120 [2]林百欣医学研究中心

出　　处：《中华肝脏病杂志》2001年第S1期40-42,共3页Chinese Journal of Hepatology

摘　　要：目的探讨脾内移植后纯化胚肝细胞的增殖能力。方法免疫吸附法纯化胚肝细胞后脾内移植，图像分析和流式细胞仪检测脾内移植后胚肝细胞的增殖。结果移植后 ３、 ７、 １４ ｄ胚肝细胞的面积密度分别为（ １６０３±３３７）μｍ２／ｍｍ２、（３７８８±６０５）μｍ２／ｍｍ２、（８１２９±１０２５）μｍ２／ｍｍ２，增殖期细胞比率为１４２５％±４．１１％、１６．０７％±５．３５％、１７．３２％±５．１７％，均明显高于成年肝细胞，差异有显著性（Ｐ＜０．０１）。结论纯化后的胚肝细胞比成年肝细胞更易于在脾内增殖。Objective To investigate whether removing the compartments of hemopoietic stem cells by immunoadsorption would improve the proliferation of fetal hepatocytes after transplantation. Methods The purification of fetal hepatocytes was performed by immunoadsorption, and the proliferative capability of fetal hepatocytes after intrasplenic transplantation was evaluated by image analysis and flowcytometry analysis. Results The yield of fetal hepatocytes after immnoadsorption was (2-3) × 107 in total per fetal liver...

关 键 词：移植 肝细胞 胚肝细胞 免疫吸附 增殖 

分 类 号：R657.3[医药卫生—外科学]