根癌农杆菌介导反义蜡质基因的水稻遗传转化  

Transformation of waxy antisense gene mediated by agrobacterium in japonica rice

作　　者：殷丽青[1] 王新其[1] 韩志勇[1] 程磊[1] 沈革志[1] 

机构地区：[1]上海市农科院作物育种栽培研究所,上海201106

出　　处：《上海交通大学学报（农业科学版）》2001年第4期285-289,共5页Journal of Shanghai Jiaotong University(Agricultural Science)

基　　金：国家科学技术部;国家转基因植物研究与产业化开发专项计划 (ZJY-A-0 2 -0 2 )

摘　　要：通过根癌农杆菌介导法 ,将水稻反义蜡质基因导入 6个水稻品种 (系 )幼胚的愈伤组织。这些愈伤组织经连续两次2 5～ 5 0 mg/L潮霉素抗性筛选 ,获得抗性愈伤组织的转化频率为 3 0 .75 %～ 5 9.0 9% ,经分化培养 ,不同品种的绿苗转化频率分别为 3 .85 %～ 8.97%。分化成苗的植株经 GUS检测 ,80 %以上的植株叶片呈阳性反应 ,显示蓝色。部分呈阴性反应的植株经 PCR检测 ,也能扩增出外源基因相应的 DNA片断 。Waxy antisanse gene was transformanted into calli which were derived from immature embryos of six rice varieties by Agrobacterium tumefaciens, these calli were cultived on selective media which containing 25～50 mg/L Hygromycin two times, 30.75%-59.09% resistant calli were obtained. These resistant calli were transferred to differentiation and regeneration media, 3.85%-8.97% calli grown up to plants. Assay of Gus activity showed that more than 80% of transgenic plants are Gus positive. A few transgenic plants which are negative for Gus assay were analysed by PCR, DNA fragments of foreign gene were detected in these plants. The results showed that foreign gene were inserted into rice plants.

关 键 词：反义蜡质基因 遗传转化 根癌农杆菌:水稻 

分 类 号：S511.032[农业科学—作物学]