苯胺降解菌的特性研究及分子生物学基础  

CHARACTERIZATION AND MOLECULAR BASIS FOR AN ANILINE-DEGRADING BACTERIUM NKS

作　　者：周霞 [1] 郑先强 [2] 佟树敏 [1] 温振国 [1] 蔡宝立 [1] 王连生 [2] 

机构地区：[1]南开大学,生命科学学院,天津,300071 [2]天津市环境保护科学研究院,天津,300191

出　　处：《南开大学学报（自然科学版）》2004年第2期24-28,33,共6页Acta Scientiarum Naturalium Universitatis Nankaiensis

摘　　要：从长期受苯胺污染环境中筛选到一株细菌NKS,能以苯胺为唯一的碳源、氮源生长.NKS降解苯胺的最适温度和pH分别为30℃和7.2,最适苯胺浓度为3000mg／L.NKS还可利用邻苯二酚和邻甲苯胺.提高接种浓度对NKS的降解效果有显著影响,重金属离子对NKS的生长和苯胺降解均有不同程度的抑制作用,以Ag+,Hg+最明显.对NKS与苯胺降解代谢有关酶类的测定结果表明,NKS中催化邻苯二酚开环的酶主要为邻苯二酚-2,3-双加氧酶(CD230),且该酶为诱导酶.PCR结果表明NKS中与苯胺酶降解有关酶的基因位于细菌的染色体上.An smiline-degrading bacteria NKS, which was able to grow on aniline as sole carbon, nitrogen and energy sources , was isolated from the nature. NKS was capable of growing on aniline medium higher than 5000mg/L. The optimun temperature and pH value for growth and aniline degradatopm were 30℃ and 7. 2, respectively. The best concentration of aniline was 3000ng/L. NKS could also use catechol and o-Toluidine as carbon, nitrogen and energy sources. but could not use Other substituted aniline compounds. A higher inoculation of bacterium could result a better degradation effect for NKS. Heavy metal ions could inhibit the growth of NKS and the degradation of aniline, especially Ag+ and Hg+. NKS degraded aniline via a meta-cleavage pathway. Only the cells growing on aniline contained catechol-2, 3-dioxygenase, which showed that catechol-2,3-dioxygenase was an inducible enzyme for NKS. PCR results indicated that genes responsible for the degradation are located on bacterial chromoaome.

关 键 词：苯胺降解酶 邻苯二酚-2 3-双加氧酶(CD23O) 质粒DNA PCR 

分 类 号：Q819[生物学—生物工程]