三种检测抗链球菌溶血素O方法的比较  

The comparison of three methds for detecting anti-streptolysin O

作　　者：张颖[1] 王艾丽[1] 江舟[1] 王娟[1] 

机构地区：[1]南京军区南京总医院解放军医学检验中心,江苏南京210002

出　　处：《医学研究生学报》2001年第z1期54-55,共2页Journal of Medical Postgraduates

摘　　要：目的 :对三种检测抗链球菌溶血素 O(ASO)的方法进行比较。　方法 :同时用自动化散射比浊法、乳胶凝集试验和溶血抑制法检测 12 0例临床患者的血清 A SO。　结果 :不同方法检测 ASO增高率 ,溶血抑制法为 44 .17%(5 3/ 12 0 ) ,比浊法为 40 .83% (49/ 12 0 ) ,乳胶凝集法为 34 .16 % (41/ 12 0 ) ,三法检测结果呈平行关系 (r=0 .988～0 .997)。 ASO水平较高 (>10 0 0 U / m l)或较低水平增高时 ,乳胶法和溶血抑制法易有误差。　结论 :比浊法检测ASO敏感性好 ,结果重复性好 ,是检测 ASO的优选方法。Objectives:To compare three methods for detecting antistrptolysin O(ASO). Methods:The ASO of in serum of 120 patients were detected by immunonephelometric method,latex agglutination test and hemolytic inhibition assay at the same time. Results:The increased rates of ASO determined by hemolytic inhibition assay,automatic nephelometry and latex agglutination test were 44.17%(53/120),40.83%(49/120),and 34.16%(41/120),respectively.the results of three methods were paralleled each other ( r =0.988~0.997).When the level of ASO was a slight or higher increasing(>1 000 U/ml),the latex agglutination test and heamolysis inhibition assay tend to be error. Conclusions:Nephelometric method is considered to be the best method for clinical determination of ASO,which is shown to be high specificity and better reproducibility.

关 键 词：ASO 比浊法 乳胶法 溶血抑制法 

分 类 号：R446.5[医药卫生—诊断学]