DNA光解酶的结构与功能研究进展  

Progress in Study on Structure and Function of DNA Photolyase

作　　者：黑晓明[1] 宋钦华[1] 郭庆祥[1] 

机构地区：[1]中国科学技术大学化学系,中国安徽合肥230026

出　　处：《生命科学研究》2001年第z1期89-96,共8页Life Science Research

基　　金：国家自然科学基金资助项目 ( 30 0 0 0 0 36 );高等学校博士学科点专项科研基金;中国科学技术大学校青年基金资助课题

摘　　要：对近年来在包括酶蛋白、辅酶的DNA光解酶结构及其与功能关系方面的研究进展作了综述 .DNA光解酶可通过光诱导的电子转移催化裂解环丁烷嘧啶二聚体 (Pyr<>Pyr) ,从而修复紫外线引起的DNA的主要损伤 .研究发现 ,来自不同有机体的光解酶均含有两个非共价的辅基 :一个是 1 ,5 二氢黄素腺嘌呤二核苷酸(FADH2 ) ,另一个是次甲基四氢叶酸 (MTHF)或 8 羟基 5 去氮杂核黄素 ( 8 HDF) ,前者具有催化活性 ,可在光作用下通过电子转移裂解嘧啶二聚体 ,后者不具有催化活性 ,但能收集光子并将能量传递给FADH2 ,具有“天线”The progress in study on structure and function of DNA photolyase was reviewed.DNA photolyase can catalyze the splitting of cyclobutane pyrimidine dimers(Pyr<>Pyr), which are the major DNA photoproducts induced by the UV component of solar radiation, via a process of photoinduced electron transfer. Photolyase are monomeric protein of 50～60 kD(1D=1u) containing stoichiometric amounts of two noncovalent cofactors. One is 1,5 dihydroflavin adenine dinucletide(FADH -) and the other cofactor is either methenyltetrahydrofolate (MTHF) or 8 hydroxy 5 deazariboflavin (8 HDF). The former has catalytic activity, via an electron transfer with the dimers, and the latter has no catalysis, but can harvest near UV vis light photon and transfer the energy to FADH - via dipole dipole coupling between two cofactors. Only apoenzyme FAD complex can bind specifically to damaged DNA substrate.

关 键 词：DNA光解酶 辅酶 环丁烷嘧啶二聚体 DNA光复活 

分 类 号：Q557[生物学—生物化学]