Experimental study on alteration of adrenergic receptors activity in neuronal membranes protein of cerebral cortex following brain trau ma in rats  

作　　者：张新伟 徐如祥 齐一龙 陈长才 

出　　处：《Journal of Medical Colleges of PLA(China)》2001年第4期292-294,共3页中国人民解放军军医大学学报（英文版）

摘　　要：Objective:To definethecourseof changestakenbyα 1 andβadrenergicreceptors(AR)activityaftertraumatic braininjury(TBI)andexploretheapproachforsecondarybraininjury(SBI)management.Methods:Theneuronalmem-braneproteinof cortexwereextractedfromtheratssubjectto traumaticbraininjury,andthechangesofα 1 -andβ-ARac-tivitiesintheneuronalmembraneswereexaminedby radioligandbindingassay(RLBA).Results:α 1 -andβ-ARactivities underwentobviouschanges,reachingtheirpeakvaluesat24h afterTBI.α 1 -ARbindingdensity(B max )reducedby22.6%whiletheligandaffinityincreasedby66.7%,andforβ-AR,however,B max increasedby116.9%andtheligandaffinityre-ducedby50.7%.Theirantagonistscouldcounteractthechangesofα 1 -andβ-ARactivity.Con clu sion:Thepatternsof changesvariesbetweenα 1 -andβ-ARactivityafterTBI, suggestingtheirdifferentrolesintheneuronalmembranesafter braintrauma,andtimelyadministrationof ARantagonistsispotentiallybeneficialinTBImanagement.Objective:To definethecourseof changestakenbyα 1 andβadrenergicreceptors(AR)activityaftertraumatic braininjury(TBI)andexploretheapproachforsecondarybraininjury(SBI)management.Methods:Theneuronalmem-braneproteinof cortexwereextractedfromtheratssubjectto traumaticbraininjury,andthechangesofα 1 -andβ-ARac-tivitiesintheneuronalmembraneswereexaminedby radioligandbindingassay(RLBA).Results:α 1 -andβ-ARactivities underwentobviouschanges,reachingtheirpeakvaluesat24h afterTBI.α 1 -ARbindingdensity(B max )reducedby22.6%whiletheligandaffinityincreasedby66.7%,andforβ-AR,however,B max increasedby116.9%andtheligandaffinityre-ducedby50.7%.Theirantagonistscouldcounteractthechangesofα 1 -andβ-ARactivity.Con clu sion:Thepatternsof changesvariesbetweenα 1 -andβ-ARactivityafterTBI, suggestingtheirdifferentrolesintheneuronalmembranesafter braintrauma,andtimelyadministrationof ARantagonistsispotentiallybeneficialinTBImanagement.

关 键 词：BRAIN TRAUMA NEURONAL membranes ADRENERGIC receptors  RATS 

分 类 号：R338[医药卫生—人体生理学]