几种影响籼稻成熟胚愈伤组织诱导及再生的因素  

Factors Influening of Callus Induction and Plantlet Regeneration from Mature Embryos in Indica Rice

作　　者：刘元风[1,2] 刘彦卓[1] 贺红[3] 王金花[1,2] 李玲[4] 李晓方 

机构地区：[1]广东省农业科学院水稻研究所 [2]华南师范大学生命科学学院,广州510631 [3]莱阳农学院农学系 [4]华南师范大学生命科学学院

出　　处：《植物生理学通讯》2004年第3期319-322,共4页Plant Physiology Communications

基　　金：国家自然科学基金重点项目 (30040025)。

摘　　要：建立了适合5个籼稻品种成熟胚籼稻遗传转化的高效植株再生体系。N6基本培养基有利于籼稻愈伤组织的诱导和继代培养 ;N6大量元素和MS微量元素有利于愈伤组织的分化。降低分化培养基中蔗糖含量,加入适量山梨醇、Cu2+、Ag+和玉米素(ZT)均可明显提高水稻愈伤组织的再生植株频率,5个品种分化频率均达到75%以上。A high efficient regeneration system of five indica rice suitable for genetic transformation wereestablished. N6 mediun was suitable for calli induction and subculture of indica rice. The N6 macroelementsand the MS microelements were advantageous to callus formation. By reducing sucrose and supplementingwith sorbitol, copper, silver and zeatin(ZT) to the media, the plantlet regeneration frequencies were evidentlyincreased. The plantlet regeneration frequencies of five varieties were over 75% in these media.

关 键 词：籼稻 成熟胚 愈伤组织 再生植株 品种 分化 

分 类 号：S511.21[农业科学—作物学]