烫伤大鼠组织ICE mRNA表达对IFN-γ影响的实验研究  

THE DYNAMIC CHANGE IN ICE mRNA EXPRESSION AND ITS EFFECT ON IFN-γ IN THERMAL INJURED RATS

作　　者：刘京衢[1] 高维谊[1] 姚咏明[1] 柴家科[1] 郭振荣[1] 盛志勇[1] 

机构地区：[1]解放军第304医院,北京100037

出　　处：《解放军医学杂志》2001年第8期567-569,共3页Medical Journal of Chinese People's Liberation Army

基　　金：国家重点基础研究发展规划项目资助课题 (编号G19990 5 42 0 3)

摘　　要：为探讨烫伤后组织白介素 1β转换酶 (IL 1βconvertingenzyme,ICE)mRNA的动态表达规律及其对IFN γ的影响 ,采用大鼠 30 %体表面积Ⅲ度烫伤模型 ,肠、肺、肝、肾等组织ICE、IL 18、IFN γmRNA含量采用逆转录聚合酶链式反应检测。结果显示 ,烫伤后 2h肠、肺、肝、肾等组织ICE、IL 18、IFN γmRNA表达较伤前值即有显著升高 ,8h达高峰 ,且一直持续至伤后 2 4h ,给予选择性消化道脱污染SDD预治疗后可不同程度抑制ICE、IL 18、IFN γmRNA的表达。提示组织ICEmRNA表达在烫伤逐渐升高 ,对IL 18、IFN γmRNA基因表达具有重要影响。To investigate the dynamic changes in ICE mRNA expression and its relationship with IL 18 and IFN γ mRNA expression in thermal injured rats.The animals were subjected to a 30% total body surface area full thickness thermal injury,and fluid resuscitation was performed at 6 hours post burn. 54 male Wistar rats were randomly divided into three groups: normal controls,30% Ⅲ degree burn group,and selective decontamination of the digestive tract (SDD) treatment group. P1asma endotoxin was measured by limulus amebocyte lysate test,and tissue ICE,IL 18 and IFN γ mRNA expression was determined by reverse transcription po1ymerase chain reaction (RT PCR).The results showed that ICE, IL 18, and IFN γ mRNA expression significantly increased in the intestine,1ung,liver,and kidney at 2 hours,peaking at 8 hours,and a high level was maintained till 24 hours. However, treatment with SDD could lower plasma endotoxin levels,and markedly inhibit ICE, IL 18, and IFN γ mRNA expression in various organs.This study suggests that major burns can lead to a marked elevation of tissue ICE mRNA expression in vital organs,which might be associated with IL 18 and IFN γmRNA expression induced by gut derived endotoxemia.

关 键 词：白介素-1β转换酶 白细胞介素-18 Γ-干扰素 基因表达 

分 类 号：R644[医药卫生—外科学]