雷公藤多甙对雄性小鼠生殖细胞毒性的研究  

Reproductive Cell Toxicity of Glycosides of Tripterygium Wilfordii Hook.f on Male Mice

作　　者：杨建一[1] 高宝珍[1] 李莉[1] 王文娟[1] 郭红刚[1] 边思成[1] 

机构地区：[1]山西医科大学细胞生物与遗传学教研室,山西太原030001

出　　处：《癌变．畸变．突变》2008年第5期393-397,共5页Carcinogenesis,Teratogenesis & Mutagenesis

基　　金：山西医科大学基础医学院"学科研究方向建设基金"(20040708)

摘　　要：背景与目的:研究雷公藤多甙对雄性小鼠生殖细胞的遗传毒性作用,为临床将雷公多甙藤作为抗生育剂和治疗药的安全使用提供理论基础和依据。材料与方法:昆明种雄性小鼠共30只,随机分成阴性、阳性对照组,雷公藤多甙抗生育剂量组[10 mg/(kg·d)]、中间剂量组[20 mg/(kg·d)]和治疗剂量组[30 mg/(kg·d)],共5组,每组6只。分别采用骨髓微核实验、精子畸形实验、染色体畸变实验和联会复合体技术,研究雷公藤多甙对雄性小鼠生殖细胞的遗传毒性。结果:雷公藤多甙20和30 mg/(kg·d)剂量组可产生体细胞遗传毒性,与阴性对照组比较差异具有统计学意义(P<0.05)。雷公藤多甙3个剂量组均可致精子畸形并对初级精母细胞产生损伤,以及具有生殖细胞联会复合体损伤,与阴性对照组比较差异均具有统计学意义(P均<0.05)。结论:雷公藤多甙具有遗传毒性和生殖细胞毒性,生殖细胞对雷公藤多甙的敏感性高于体细胞,作为治疗药物忌大剂量长时间使用。BACKGROUND AND AIM: In order to detect glycosides of tripterygium wilfordii Hook.f(GTW)male mice genital system toxicity,which can provid rationale and evidence to use safely during the period of clinical treatment.MATERIALS AND METHODS:30 Kunming male mice were randomly divided into five groups with 6 mice in each,three dosage groups(antifertility-dosage groups [10 mg/(kg·d)],medium-dosage groups[20 mg/(kg·d)] and remedy-dosage groups[30 mg/(kg·d)],one negative control and one positive control group.This essay introducts micronucleus of bone marrow,sperm deformity,chromosome aberration,and analyze of synaptonemal complex,approaching effect of GTW to male mice in genesis toxicity.RESULTS: Micronuclei of bone marrow cell indicates 20 mg/(kg·d) group and 30 mg/(kg·d) group of GTW generate somatocyte inherent toxicity,were significantly higher than that of negative control.Every-dosage group of GTW cause sperm deformity,do harm to primary spermatocyte chromosome aberration and synaptonemal complex,were significantly higher than that of negative control.CONCLUSION: GTW had somatocyte inherent and reproductive cell toxicity,reproductive cell was higher sensitivity than somatocyte,which cann′t apply under large-dosage for a long time as the antifertility or the therapy drug.

关 键 词：雷公藤多甙 骨髓微核 精子畸形 染色体畸变 联会复合体 

分 类 号：R992[医药卫生—毒理学]