新疆伊犁地区马的尼帕病毒感染情况研究  

Research on Nipah virus infection in equine of Yili, Xinjiang Uygur Autonomous Region

作　　者：刘妍汐[1,2] 谢鹏[1,2,3] 曾志磊[1,2] 翟红[1,2] 徐鸣明[1,2] 张英英[1,2] 余建萍[1,2] 陈晓[1,2] 彭丹[1,2] 朱丹[1,2] 

机构地区：[1]重庆医科大学附属第一医院神经内科,重庆400016 [2]重庆医科大学基础研究所 [3]感染性疾病分子生物学教育部重点实验室

出　　处：《重庆医科大学学报》2008年第z1期27-29,共3页Journal of Chongqing Medical University

基　　金：国家高技术研究发展计划(863计划)(2006AA02Z196)

摘　　要：目的:探讨尼帕病毒(Nipah virus,NiV)在新疆伊犁地区马群中的自然感染情况。方法:采用尼帕病毒一步法实时定量逆转录聚合酶链反应(One step real time reverse transcriptase polymerase chain reaction,Real-time RT-PCR)检测120匹马外周血液单个核细胞(Peripheral blood mononuclear cells,PBMCs)中的NiVN基因片段,对阳性产物进行基因序列测定、同源性和氨基酸顺序分析。结果:所检测120匹马PBMCsNiVN基因片段阳性率为0%(0/120)。结论:本研究未发现新疆伊犁地区马中存在尼帕病毒自然感染。Objective:To investigate the epidemics of Nipah virus(NiV)in Yili, Xinjiang Uygur Autonomous Region. Methods:One step real time reverse transcriptase polymerase chain reaction(real-time RT-PCR)was performed to detect the NiV N in peripheral blood mononuclear cells from 120 equines. Gene sequence and amino acid sequence were analyzed for positive products.Results:The positive rate of NiV N in PBMCs from 120 equines was 0%(0/120).Conclusion:The investigation do not support that NiV infection exist in Yili, Xinjiang Uygur Autonomous Region.

关 键 词：尼帕病毒 REAL-TIME RT-PCR 一步法 TAQMAN探针 

分 类 号：R51[医药卫生—内科学]