病毒性脑炎患者血液和脑脊液NSE含量测定及其与临床的相关性研究  

Assay of NSE concentration in serum and cerebrospinal fluid of patients with viral encephalitis and its clinical correlation

作　　者：王振海[1] 陈建红[1] 马斌武[1] 谢鹏[2] 孔繁元[1,3] 

机构地区：[1]宁夏医学院附属医院神经内科宁夏颅脑疾病重点实验室 [2]重庆医科大学附属第一医院神经内科 [3]重庆医科大学附属第一医院神经内科,重庆400016

出　　处：《重庆医科大学学报》2008年第z1期60-62,77,共4页Journal of Chongqing Medical University

基　　金：2006年国家"863"计划项目(编号:2006AA02Z196);2007年国家自然科学基金面上项目(编写:30760067);2007年宁夏自然科学基金项目(编号:NZ0790);2007年第四十二批中国博士后科学基金项目(编号:20070420721)

摘　　要：目的:为了观察病毒性脑炎急性期和恢复期患者血液和脑脊液的NSE含量变化,探讨其与异常脑电图和影像学低密度灶体积等方面的相关性。方法:将42例病毒性脑炎住院患者作为观察组,25例与观察组相匹配的阑尾或胆囊摘除手术病人以及健康体检者作为对照组;采用ELISA法,检测其血液及脑脊液NSE浓度;并应用SPSS13.0统计软件包进行统计学分析。结果:病毒性脑炎急性期患者血液和脑脊液NSE浓度显著高于恢复期患者和对照组(P<0.01);血液和脑脊液NSE浓度与低密度灶体积均呈显著正相关(rcsf=0.736,P<0.01;r血液=0.685,P<0.01);轻度、中度及重度异常脑电图患者脑脊液NSE浓度均高于血液(P<0.01);重度、中度异常脑电图患者血液及脑脊液NSE浓度均高于轻度(P<0.01)。结论:脑脊液或血清中的NSE浓度,反映了神经细胞和神经胶质细胞的损害程度,也是脑组织破坏后较合适的生化标记物。Objective:To investigate the changes of neuron-specific enolase(NSE)concentration in serum and cerebrospinal fluid from patients with acute and recovery viral encephalitis and to find out its correlation with anomaly electroencephalogram and low density focus volume in imageology. Methods:42 cases of patients with viral encephalitis were enrolled and 25 cases of health examinater or patients with operation of appendix or cholecyst were enrolled in control group.We measured the NSE concentration in cerebrospinal fluid and serum using enzyme-linked immunosorbent assay. Comparison analysis was performed by using a SPSS13.0 statistical package. Results:NSE concentration in serum and cerebrospinal fluid of patients with acute viral encephalitis was significantly higher than that of recoverys and control group(P<0.01). The release of NSE concentration in serum and cerebrospinal fluid of patients with acute viral encephalitis was associated with the low density focus volume of imageology(rcsf=0.736,P<0.01; rblood=0.685,P<0.01). NSE concentration in cerebrospinal fluid was significantly higher than that in serum from patients with light, midrange and severe anomaly electroencephalogram(P<0.01). Conclusion:The NSE concentration in serum and cerebrospinal fluid from patients with acute viral encephalitis indicates the damage degree of neuroglial lesion. It is a useful biochemical marker for brain-tissue damage.

关 键 词：神经元特异性烯醇化酶 病毒性脑炎 酶联免疫吸附法 

分 类 号：R512.3[医药卫生—内科学]