苏木乙酸乙酯提取物对MsPGN模型大鼠肾组织TNF-α mRNA表达的影响  

Effect of ethyl acetate extract from sappan wood on the expression of TNF-α mRNA in nephridial tissue of MsPGN rats

作　　者：郑佳新[1] 周亚滨[2] 刘颖哲[2] 客蕊[2] 陈会君[2] 孙晶[2] 王岩[2] 

机构地区：[1]黑龙江省中医研究院肾内科,哈尔滨150036 [2]黑龙江中医药大学,哈尔滨150000

出　　处：《现代免疫学》2008年第5期399-403,共5页Current Immunology

基　　金：黑龙江省科学计划攻关项目(20010101002-00)

摘　　要：观察苏木乙酸乙酯提取物对MsPGN模型大鼠肾组织TNF-α mRNA表达,探讨苏木免疫抑制作用机制。将40只大鼠随机分成四组,每组10只,复制邹氏[1]改良的慢性血清病MsPGN动物模型,空白组和模型组每日灌服按体重计算等容量橄榄油;雷公藤组给予雷公藤多甙片橄榄油灌胃,6.5 mg/kg.d;苏木组给予苏木橄榄油灌胃,1.15 g/kg.d(含生药量);观察相关指标。结果显示各组电泳条带亮度有明显差异,模型组与空白组比较(P<0.01);与模型组相比较,苏木组、雷公藤组肾脏中的TNF-α mRNA表达减少,苏木组(P<0.01),雷公藤组(P<0.05);而苏木组与雷公藤组比较无显著性差别(P>0.05)。综上可见苏木乙酸乙酯能够抑制大鼠MsPGN模型肾脏中的TNF-α mRNA表达,减轻其病理损伤,并有利尿消肿和降低蛋白尿的作用。To observe the effect of ethyl acetate extract from sappan wood on the expression of TNF-α mRNA in nephridial tissue of MsPGN rats in order to explore the mechanism of its immunosuppressive action.The modified animal model of chronic serum sickness advocated by Zou et al was reproduced in 40 Wistar rats and they were randomly divided into 4 groups,each group containing 10 rats;i.e.blank group,model group,Tripterygll wilfordii group and sappan wood group.The dosages calculated by body weight used for T.wilfordii and sappan wood and administrated with equal amount of olive oil through gastric route were 6.5 mg/kg·day and 1.15 g/kg.day(including the crude drug).respectively.The RT-PCR products were analyzed by agarose electrophoresis and Kodak 2.0 software.It was found that significant difference in the brightness of electrophoretic bands in various groups was obvious.Compared with the blank group,the expression of TNF-α mRNA in the model group was significantly increased(P<0.01).however,in comparison with that of the model group,those in the nephridial tissues of sappan group and T.wilfordii group were significantly reduced(P<0.01 and P<0.05).No significant difference in the expression of TNF-α mRNA could be demonstrated between sappan group and T.wilfordii group(P>0.05).Nevertheless,there were evidences to indicate that the pathological damages in these two groups were decreased.From these observations,it is evident that the ethyl acetate extract from sappan wood can inhibit the expression of TNF-α mRNA in the nephridial tissues of MsPGN rats and lighten the pathological damages including diuresis to reduce edema and albuminuria.

关 键 词：苏木 活血化淤 免疫抑制 系膜增生性肾炎 

分 类 号：R392.4[医药卫生—免疫学]