HPLC同时检测保健品及中成药中非法添加9种抗高血压化学药物的研究  

Study on the Method for Separation and Identification of 9 Antihypertensives by HPLC

作　　者：刘吉金[1] 杨敏[1] 李军[1] 王铁杰[1] 

机构地区：[1]深圳市药品检验所,广东深圳518029

出　　处：《中国现代应用药学》2008年第z2期717-719,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 建立一种RP-HPLC-DAD 条件可同时对降压保健品及中成药中非法添加的9种化学合成降压药(阿替洛尔、氢氯噻嗪、盐酸可乐定、卡托普利、酒石酸美托洛尔、盐酸普奈洛尔、盐酸地尔硫卓、利血平、苯磺酸氨氯地平)进行检查的方法.方法 采用C18(250 mm×4.6 mm,5 μm)色谱柱,甲醇为流动相A,0.03 moL·L-1磷酸二氢钾溶液(用磷酸调pH值至3.0)为流动相B,进行梯度洗脱;流速:0.7 mL·min-1;柱温:35 ℃;二极管阵列检测器,检测波长为215 nm,并同时采集200～400 nm的光谱图.结果 在本色谱条件下,9种降压药有很好的分离度,最小检出量阿替洛尔、盐酸可乐定、卡托普利、苯磺酸氨氯地平、酒石酸美托洛尔为25 ng,氢氯噻嗪、盐酸地尔硫卓为10 ng,盐酸普奈洛尔、利血平为5 ng.结论 本法简便准确,可作为降压保健品及中成药中非法添加化学成分的快速筛查方法.OBJECTIVE To establish a RP-HPLC for the simultaneous separation and identification of 9 antihypertensives(Atenolol,Hydrochlorothizide,Clonidine Hydrochloride,Captopril,Metoprolol Tartrate,Propranolol Hydrochloride,Diltiazem Hydrochloride,Reserpine,Amlodipine Besylate)which were illegally added into the the products of health care and Chinese traditional patent medicines.METHODS The chromatography system consisted of C18(250 mm×4.6 mm,5μm)column and mobile phase consisted of methanol-0.03 moL·L-1 potassium dihydrogen phosphate(using phosphoric acid to adjust pH to 3.0) with linear gradient elution.The flow rate was 0.7 mL·min-1.The column temperature was 35 ℃ and the detection wavelength was set at 226 nm and simultaneously collected the spectrum at wavelength of 200-400 nm.RESULTS On the condition of the selected chromatogram,there was a good resolution among 9 antihypertensives,and the minimum detection quantity of Atenolol,Clonidine Hydrochloride,Captopril,Amlodipine Besylate,Metoprolol Tartrate were 25 ng,and Hydrochlorothizide,Diltiazem Hydrochloride were 10 ng,Propranolol Hydrochloride,Reserpine were 5 ng.CONCLUSION This method is simple,accurate and can be employed in the examination of chemical medicine in the products of health care and Chinese traditional patent medicine for Antihypertensives.

关 键 词：保健品 中成药 非法添加 抗高血压药物 高效液相色谱法 

分 类 号：R917.101[医药卫生—药物分析学] R927.12[医药卫生—药学] R972.4