HPLC测定复方首乌降脂胶囊中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷的含量  

Determination of 2,3,5,4'-tetrahyroxy-tilbene-2-O-β-D glucoside in Gold Theragran Capsules by HPLC

作　　者：刘杰[1] 郑思嘉[2] 韩金玟 胡海燕[2] 

机构地区：[1]中山大学附属第三医院,广州510630 [2]中山大学药学院,广州510080 [3]广东药学院

出　　处：《中国现代应用药学》2008年第z2期719-721,共3页Chinese Journal of Modern Applied Pharmacy

摘　　要：目的 建立复方首乌降脂胶囊中2,3,5,4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷(TTG)的含量测定方法.方法 采用HPLC法,Diamomsil C18柱(4.6 mm×250 mm,5 μm);流动相为甲醇-6.8×10-3mol·L-1 pH 3磷酸盐缓冲液(36∶64);柱温35 ℃;流速1 mL·min-1;检测波长320 nm.结果 TTG在0.224～3.36 μg内呈良好的线性关系(n=6, r=1.000 0),加样回收率为98.24%,RSD为2.61%.结论 该方法灵敏、准确、简便,可用于该制剂的质量控制.OBJECTIVE To develop a method to determine 2,3,5,4'-tetrahyroxy-tilbene-2-O-β-D glucoside in Gold Theragran Capsules.METHODS HPLC conditions: A Diamomsil C18 column,a mixture of methanol-6.8×10-3mol·L-1 pH 3 phosphate buffer(36∶64) as mobile phase,column temperature 35 ℃,flow rate 1 mL·min-1 and UV detection wavelength at 320 nm.RESULTS The linear range of calibration curve was 0.224~3.36 μg(n=6) with the correlation coefficient of 1.000 0,the average recovery with RSD being(98.24±2.61)%.CONCLUSION The method is sensitive,accurate and speedy,therefore suitable for product's quality control

关 键 词：复方首乌降脂胶囊 2 3 5 4'-四羟基二苯乙烯-2-O-β-D-葡萄糖苷 HPLC 

分 类 号：R917.101[医药卫生—药物分析学] R972.6[医药卫生—药学]