标本因素对荧光定量PCR法测定乙肝病毒DNA的影响  

作　　者：郑建中[1] 王丽萍[1] 

机构地区：[1]山东邹城兖矿集团总医院,273500

出　　处：《中国医药指南》2008年第17期431-433,共3页Guide of China Medicine

摘　　要：目的探讨脂血、溶血标本及血清标本常温储存48小时和4℃保存7天对乙肝病毒DNA(HBVDNA)荧光定量测定结果的影响。方法:将乙肝大三阳血清标本进行即时检测、412保存7d后检测、室温保存48h检测及高脂血和非脂血、溶血血清和未溶血血清同时作HBVDNA荧光定量PCR,并两两间进行配对t检验。结果乙肝大三阳溶血与未溶血血清标本、脂血和非脂血血清标本HBVDNA含量都在同一数量级。血清标本在室温下短期贮存(48h内)、4℃保存7d内分别与即时检测HBV-DNA含量比较均无统计学意义(P>0.05)。结论脂血、溶血,血清标本不同储存温度及时间对HBV-DNA定量结果测定无影响。Objective:To investigate the effect of sample hemolysis,the high lipid,sera storage at 4℃for 7 days and storage at room temperature on fluorimetric quantification of HBV-DNA by real=time PCR detection.Method:The serums from the case that were HBsAg+,HBeAg+ and anti-HBc+,the serums from the case first detections were performed as soon as the blood was drawn.The second and the third detections were done after the blood was stored at 4℃for 7 days and at room temperature.The results of three detections were compared one another statistically;the serums were high lipids or hemolytic blood were tested by real-time fluorimetry PCR of HBVDNA parallel.The data were treated with rank sum test statistics.Results:9 samples of the hemolytic blood and 9 high lipids have no siginificant change compared with the contrast in HBVDNA:the serum specimens at 4℃for less than 7 days and at room temperature aslo showed no statistical significance.Conclusion:To store the serum specimens at 4℃for less than 7 days and at room temperature,the fluorimetric quantification of HBV-DNA by PCR detection will not be affected:the hemolytic blood and the serum with high lipid also have no affecting to the real time fluorimetry PCR of HBVDNA.

关 键 词：Real-time fluorimetry PCR of HBVDNA SAMPLE 

分 类 号：R512.62[医药卫生—内科学]