中孕期小鼠CD4^+Foxp^(3+)调节性T细胞的比例变化  

Proportion change of the CD4^+Foxp^(3+) regulatory T cells in the mid-gestation of mice

作　　者：昌盛[1,2] 汪理[1,2] 林星光[1,2] 金昊[1,2] 童荔[1,2] 陈忠华 

机构地区：[1]华中科技大学同济医学院附属同济医院器官移植研究所 [2]国家教育部/卫生部重点实验室,武汉430030

出　　处：《现代免疫学》2008年第6期452-456,共5页Current Immunology

基　　金：国家自然科学基金面上资助项目(30500468)

摘　　要：本实验通过检测中孕期小鼠全身及局部CD4+Foxp3+调节性T细胞的比例变化,探讨妊娠状态下CD4+Foxp3+调节性T细胞与异基因胎儿抗原刺激之间的相互关系。将成熟B ALB/c雌鼠与BALB/c雄鼠或C57BL/6雄鼠交配作为实验组,选取未孕BALB/c雌鼠为对照组。受孕10~12 d后分离小鼠的外周血、脾脏、淋巴结、胎盘,分别用流式细胞学、免疫组化、RT-PCR法检测相关组织中的CD4+Foxp3+调节性T细胞的变化。结果:(1)流式检测实验组中同基因孕鼠和异基因孕鼠脾脏的CD4+Foxp3+T细胞占CD4+T细胞的比例较对照组明显增高(P<0.01);但是同基因和异基因孕鼠之间这类细胞比例的变化并无明显差异(P>0.05)。同样,在淋巴结和外周血中也取得类似结果;(2)免疫组化S-P法显示对照组和实验组均可在脾脏、髂淋巴结组织中表达Foxp3,但是对照组Foxp3+调节性细胞的数目显著低于实验组(P<0.01),但是同基因和异基因妊娠组间无明显差异(P>0.05);(3)RT-PCR法从同基因孕鼠和异基因孕鼠的胎盘局部检出Foxp3 mR-NA的表达。中孕期小鼠全身相关组织中CD4+Foxp3+调节性T细胞的比例显著增高,其扩增并非由于父系来源的MHC抗原所致,而是由妊娠本身所介导。另外,在胎盘局部有Foxp3 mRNA的高表达,由此推测CD4+Foxp3+调节性T细胞可能在母胎界面的局部发挥作用,抑制针对胎儿的免疫排斥。The interrelationship between the regulatory T cells and the antigenic stimulation of fetus was investigated by detecting the proportion change of the CD4+Foxp3+ regulatory T cells during mid-gestation of mice..In the present study,the proportion change of the CD4+Foxp3+ regulatory T cells in tissues of pregnant BALB/c mice at gestation day 10.5(E10.5) during which the female mice had been successively mated with male BALB/c or C57BL/6 mice was qualified by using flow cytometry analysis immunohistochemical ...

关 键 词：妊娠 母胎耐受 CD4+Foxp3+调节性T细胞 FOXP3 

分 类 号：R392.1[医药卫生—免疫学]