膜型B7-H1分子诱导产生的T抑制细胞及其免疫学特性的初步研究  

The suppressor T cells induced by membranous B7-H1 molecule and their immunobiological properties

作　　者：路丽明[1] 丁庆[1] 杨能[1] 周晓荣[1] 周芸[1] 周光炎[1] 

机构地区：[1]上海交通大学医学院上海市免疫学研究所,上海200025

出　　处：《现代免疫学》2008年第6期465-469,共5页Current Immunology

基　　金：教育部博士点基金资助项目(20060248084);上海市选拔优秀青年教师资助计划;上海市教委基金资助项目(2685619)

摘　　要：为了探讨B7-H1分子诱导产生的T抑制细胞免疫生物学特性,我们首先构建了人B7-H1基因,筛选出细胞膜表面稳定表达B7-H1蛋白的人ECV304细胞系,并在体外建立了由这种非专职抗原提呈细胞系刺激诱导纯化的CD4+T细胞,并使其具有抑制功能的新方法。通过检测其增殖效应和上清细胞因子水平的变化,观察到这群细胞对同种异体混合淋巴细胞反应(MLC),具有明显抑制作用,该作用与大量产生IL-10、TGF-β细胞因子有关。实验表明,ECV304/B7-H1可有效诱导具有免疫抑制的功能T细胞,为其进一步应用于临床治疗打下基础。To explore the mechanism of the membranous B7-H1 molecule acting as an immunosuppression factor,the human B7-H1 gene was at first constructed and then transfected to human ECV304 cell line derived form human umbilical vein to screen the stably expressed B7-H1 molecules on cell surface.The purified CD4+T cells induced by this non-professional antigen-presenting cell line were established in vitro.and their effective suppressive effect of these cells to allogenic MHC was observed by testing their proliferativ...

关 键 词：B7-H1 ECV304 免疫抑制 T抑制细胞 

分 类 号：R392.12[医药卫生—免疫学]