NOD小鼠自然状态下CD4^+CD25^+T细胞的动态变化及意义  

Dynamic change of CD4^+CD25^+ T cells in non-obese diabetic mice under natural condition

作　　者：张梅[1] 徐书杭[1] 茅晓东[1] 刘翠萍[1] 刘超[1] 

机构地区：[1]南京医科大学第一附属医院内分泌科,南京210029

出　　处：《现代免疫学》2008年第6期479-482,共4页Current Immunology

基　　金：江苏省政府医学重点学科项目基金资助项目(苏卫科教2001-34)

摘　　要：研究自发的1型糖尿病雌鼠模型(NOD)在自然状态下发生1型糖尿病过程中CD4+CD25+T细胞的动态变化,旨在初步探讨调节性T细胞参与1型糖尿病发病的可能机制。采用雌性NOD小鼠作动物模型,每2周尾静脉采血1次,采用三色流式细胞术测定NOD小鼠外周血中CD4+CD25+T细胞(CD3+CD4+CD25+)的百分率。在32周时,对比发生糖尿病和未发生糖尿病NOD小鼠不同脏器中的CD4+CD25+T细胞阳性率。HE法检测胰岛炎。结果显示:(1)自第6周起NOD小鼠CD4+CD25+T细胞百分率逐渐降低。发生糖尿病NOD小鼠CD4+CD25+T细胞比率低于未发病NOD小鼠对照组(外周血分别为0.94%±0.21%、1.62%±0.23%,P=0.01;脾脏2.09%±0.14%、2.77%±0.36%,P=0.019),提示糖尿病NOD小鼠外周血中存在异常比例的CD4+CD25+T细胞;(2)32周龄糖尿病NOD小鼠与未发病NOD小鼠的CD4+CD25+T细胞抑制功能减低,与阳性对照组有显著性差异;(3)HE染色结果示糖尿病NOD小鼠胰岛结构完全破坏,胰岛炎程度较未发病NOD小鼠严重。该结果提示NOD小鼠发生糖尿病时免疫功能紊乱与CD4+CD25+T细胞参与调节及T细胞亚群变化相关,糖尿病的发生受致病性T细胞和调节性T细胞的调节。To investigate the dynamic change of CD4+CD25+ T cells in non-obese diabetic mice(NOD) to develop type 1 diabetes mellitus and explore the possible mechanism of the regulatory T cells participating the pathogenesis of diabetes,the percentage of CD3+CD4+CD25+ T cells in peripheral blood of female NOD mice was determined every 2 weeks with TC-CD3,FITC-CD4 and PE-CD25 antibodies staining and analyzed by flow cytometry.Spleen cell suspensions were enriched for CD4+ T cells by panning and were sorted on FACS int...

关 键 词：CD4+CD25+T细胞 1型糖尿病 NOD小鼠 

分 类 号：R392.11[医药卫生—免疫学]