RP-HPLC荧光法测定兔血浆中BWYJ的浓度及药代动力学参数测定  

Determination of naftopidil'ramification-BWYJ concentration in rabbit plasma by RP-HPLC with fluorescence detector

作　　者：杨丹莉[1] 黄林[1] 袁牧[2] 吴芹[1] 陆远富[1] 聂晶[1] 谢笑龙[1] 

机构地区：[1]遵义医学院药理学教研室贵州省基础药理重点实验室,贵州遵义563003 [2]贵州省中科院天然产物重点实验室,贵州贵阳550002

出　　处：《遵义医学院学报》2008年第5期511-514,共4页Journal of Zunyi Medical University

基　　金：2005贵州省卫生厅优秀青年人才基金资助

摘　　要：目的建立了RP-HPLC-荧光法检测兔血浆中BWYJ浓度。方法以C18色谱柱(150mm×416mm,5μm)分离;以盐酸普萘洛尔为内标,流动相为以甲醇-乙腈-磷酸缓冲液(28.5:28.5:43);流速:1ml.min-1,柱温:37℃;荧光检测器,激发波长229nm,发射波长335nm。结果日间RSD≤3.26%,日内RSD≤2.14%,平均相对回收率为101.50%±5.72%;BWYJ的保留时间为10.65±0.50min,内标的保留时间为3.87±0.31min。血药浓度在0.05～20μg.ml-1范围内关系良好(r=0.99),最低检测限为5ng。兔单次静脉注射BWYJ5mg.kg-1后,T1/2α、T1/2β分别为1.24±0.21min,42.70±9.43min。结论该法快速、灵敏、准确,可用于BWYJ的体内分析。Objective To establish a HPLC-FLD method for determining the concentration of BWYJ in rabbit plasma.Methods The sample was separated with a C18 column(150mm ×416mm,5μm) at 37℃ with the mobile phase methanol-acetonitrile-phosphate buffer(28.5:28.5:43).A fluorescence detector was used(λex = 229 nm,λem = 335 nm);The flow rate was 1 ml.min-1.Propranolol was used as the internal standard.Results The retention time of BWYJ was(10.65± 0.5) min and the retention time of Propranolol was(3.87±0.31) min;A good linear ...

关 键 词：萘哌地尔衍生物BWYJ HPLC荧光检测法 药代动力学 

分 类 号：R96[医药卫生—药理学]