原花青素对·OH致损的鼠肝线粒体的保护作用  

Effects of Procyanidins on Preventing Mitochondria Damage in Rat Liver Induced by ·OH

作　　者：陈季武[1] 朱振勤[1] 苏裕[1] 王妮[1] 曹家琪[1] 刘国伟[1] 胡斌[1] 翟万垠[2] 

机构地区：[1]华东师范大学生命科学学院,上海200062 [2]中国科学院硅酸盐研究所生物材料与组织工程中心,上海200050

出　　处：《华东师范大学学报（自然科学版）》2004年第2期92-96,共5页Journal of East China Normal University(Natural Science)

摘　　要：采用分光光度法和荧光偏振法,检测了原花青素对·OH引起的鼠肝线粒体损伤的保护作用。结果表明,原花青素能有效增加受损伤的线粒体的膜脂流动性,明显减少受损伤的线粒体肿胀及脂质过氧化物的形成,从而维护线粒体结构和功能的完整性。以上结果为原花青素的进一步开发提供了新的资料。Oxidative damage of the mitochondria plays an important role in generating and developing process of many diseases. Hydroxyl radical (·OH) has been considered an important factor inducing mitochondria damage. Therefore, it has important significance to study and exploit medicines protecting mitochondria damage caused by ·OH. We measured effects of procyanidins preventing mitochondria damage induced by ·OH using spectrophotometry and fluorescent polarimetry. The results showed that procyanidins increased effectively membrane lipids fluidity of damaged mitochondria,decreased significantly the mitochondria swelling and lipid peroxidation product formation. As a result,procyanidins can maintain the integrity of structure and function of mitochondria. Above results can provide new data for further exploitation of procyanidins.

关 键 词：原花青素 羟自由基 线粒体 线粒体肿胀 脂质过氧化 膜脂流动性 

分 类 号：Q505[生物学—生物化学]