建立PC-PCR法快速、半定量检测养殖环境和水产动物中的致病性副溶血弧菌  

Rapid and Semi-quantitative Detection of Pathogenic Vibrio paraharmolyticus from Environment and Aquatic Animals by PC-PCR

作　　者：马妍[1] 李健[2] 王群[2] 何玉英[2] 王斌[1] 

机构地区：[1]大连水产学院生命科学与技术学院,辽宁大连116023 [2]中国水产科学研究院黄海水产研究所农业部海洋渔业资源可持续利用重点开放实验室,山东青岛266071

出　　处：《中国农业科技导报》2008年第S1期67-72,共6页Journal of Agricultural Science and Technology

基　　金：国家863计划项目(2006AA10A406);国家科技支撑计划项目(2006BAD01A13);公益性农业行业科研专项(nyhyzx07-042)资助

摘　　要：以tl为靶基因,将菌落PCR技术和平板菌落计数法相结合,建立致病性副溶血弧菌快速、半定量检测方法。PCR扩增的目的片段为673 bp,人工污染样品检测最低限固体样品为3.1×102cfu/g,液体样品为1.3×102cfu/mL,菌落PCR每反应体系的检测低限为90 cfu。对人工污染样品的检测结果显示,PC-PCR方法检测结果与常规检测结果一致,可用于养殖环境及水产品中细菌的动态监测。A rapid and semi-quantitative method was developed for detection of pathogenic Vibrio paraharmolyticus,using tl gene as a targeted gene,combined the method of plate count for bacterial colonies and colony PCR.The expected PCR product was 673 bp.Detection limit for artificial contaminants was 3.1×102 cfu/g of solid sample and 1.3 102 cfu/ml of liquid sample;Detection limit of colony PCR reaction system was 90 cfu.The contaminated samples were detected by both PC-PCR and routine methods with a completely coin...

关 键 词：副溶血弧菌 快速检测 半定量 菌落PCR tl基因 

分 类 号：S941[农业科学—水产养殖]