影响体胚发生途径香蕉(Musa spp.,AAB Group)植株再生的因素  

Factors Affecting Banana (Musa spp., AAB Group) Plant Regeneration via Embryogenesis

作　　者：徐春香[1] Bart PANIS Hannelore STROSSE Rony SWENNEN 李华平[1] 肖火根[1] 范怀忠[1] 

机构地区：[1]华南农业大学植物病毒研究室 [2]比利时荷语鲁汶大学热带作物改良实验室

出　　处：《植物生理学通讯》2004年第3期293-296,共4页Plant Physiology Communications

基　　金：国家自然科学基金(39870434);广东省自然科学基金(990714);广东省科技攻关(C20209)。

摘　　要：香蕉品种‘Agbagaba’和‘Orishele’的胚性细胞悬浮系(ECS)在液体培养基中分别预培养1和2周后,将其接种在RD1和M3培养基上,于光照或黑暗条件下进行体胚的再生。从沉积细胞体积(SCV) 为1 mL(1 mL SCV)的ECS获得的再生体胚数量因预培养时间、再生培养基的种类及培养条件的不同而异。植株的再生率及从1 mL SCV的ECS获得的再生植株数量也受上述体胚再生条件的间接影响。Embryogenic cell suspensions(ECSs) of Agbagaba and Orishele (Musa spp., AAB Group) wereused to regenerate embryos after 1-week and 2-week pre-culture in liquid medium respectively. The ECSs wereinoculated on regeneration medium RD1 or M3 and incubated under light or in dark. The amount of regenerablesomatic embryos that could be regenerated from per milliliter settled cell volume (SCV) of ECS depended onpre-culture time in liquid media, the types of regeneration media and incubation conditions. Plant regenerationfrequency from the somatic embryos and plantlets could be regenerated from 1 mL SCV of ECS were indirectlyaffected with those conditions for the regeneration of somatic embryos mentioned above.

关 键 词：香蕉 体胚发生 体胚再生 植株再生 培养基 

分 类 号：S668.1[农业科学—果树学]