不同频率张应变作用下血管磷酸化HSP27的表达及其在血管平滑肌细胞迁移中的作用  

Expression of p-HSP27 in vessel under different frequencies of cyclic tensile strain and its role in the migration of vascular smooth muscle cells

作　　者：李振坤[1] 周瑾[1] 刘波[1] 齐颖新[1] 沈宝荣[1] 姜宗来[1] 

机构地区：[1]上海交通大学力学生物学与医学工程实验室,上海200240

出　　处：《医用生物力学》2008年第6期415-419,433,共6页Journal of Medical Biomechanics

基　　金：国家自然科学基金资助项目(30570459;10732070)

摘　　要：目的研究血管和血管平滑肌细胞(vascular smooth muscle cells,VSMCs)磷酸化热休克蛋白27(heat shock protein27,HSP27)在不同频率张应变条件下的表达变化及其对VSMCs迁移的影响。方法应用血管体外应力培养系统,在频率为0.5Hz和1.25Hz、平均压力15.996 kPa(120mmHg)和平均流量50mL/min的条件下培养猪颈总动脉,培养时间为24h;又用Flexercell细胞应变加载系统,对人VSMCs施加0.5Hz和1.25Hz频率的10%张应变,加载时间为12h。然后,Western blot检测不同频率张应变作用下血管和VSMCs的磷酸化HSP27的表达变化。应用RNA干扰技术,分别观察抑制HSP27表达前后的VSMCs迁移能力变化。结果与频率为0.5Hz作用相比,1.25Hz张应变可以激活血管和VSMCs的HSP27表达,抑制VSMCs迁移。RNA干扰HSP27后,VSMCs迁移数量明显增多。结论正常生理频率张应变可能通过激活血管HSP27抑制VSMCs迁移,以维持血管正常的结构和功能。Objective To investigate the expression of p-HSP27 in vessel and vascular smooth muscle cells(VSMCs) exposed to different frequencies of cyclic tensile strain respectively, and to study its role in the migration of VSMCs.Methods Porcine common carotid arteries were cultured in a blood vessel organ-culture system under pulsatile flow for 24 hours.The frequencies of the flows studied were 0.5 Hz and 1.25 Hz.The mean pressure and flow rate were 15.996 kPa(120 mmHg) and 50 ml/min respectively.Human VSMCs were a...

关 键 词：张应变 频率 血管平滑肌细胞 HSP27 细胞迁移 RNA干扰 

分 类 号：R318.01[医药卫生—生物医学工程]