通心络对晚期糖基化终产物诱导的内皮祖细胞存活的影响及其机制  

Effects of Tongxinluo on survival of endothelial progenitor cells exposed to advanced glycation end products and the possible mechanism

作　　者：孙承波[1,2] 梁春[1] 吴宗贵[1] 任雨笙[1] 潘晓明[1] 贺治青[1] 

机构地区：[1]第二军医大学附属长征医院心内科,上海200003 [2]江苏省连云港市第一人民医院心内科

出　　处：《上海医学》2008年第10期711-714,共4页Shanghai Medical Journal

基　　金：国家重点基础研究发展计划(973计划)(2005CB523309)资助项目

摘　　要：目的探讨通心络对晚期糖基化终产物(AGEs)诱导的内皮祖细胞(EPCs)存活的影响及相关的分子机制。方法分离出人外周血单个核细胞后经体外诱导分化为EPCs,采用Western印迹法检测通心络对糖基化人血清白蛋白(AGE-HSA)刺激的EPCs的丝裂原活化蛋白激酶(MAPK)通路以及AGEs受体(RAGE)表达的影响,同时观察不同浓度通心络对AGE-HSA刺激的EPCs增殖及凋亡的影响。结果通心络超微粉溶液可抑制由AGE-HSA诱导的EPCs MAPK信号通路的激活,其中以100mg/L的通心络对p38和细胞外信号调节酶(ERK)MAPK信号通路的抑制作用最为显著(P值均<0.01)。结论通心络主要通过抑制AGE-HSA诱导EPCs MAPK(主要是p38和ERK)信号通路的激活,下调EPCs RAGE的表达,进而抑制EPCs的凋亡并促进其增殖。Objective To investigate the effects of Tongxinluo (TXL) on the survival of endothelial progenitor cells(EPCs) exposed to advanced glycation end products(AGEs) and the related molecular mechanism.Methods Human peripheral blood mononuclear cells were separated by density gradient centrifugation and were induced to differentiate into EPCs in vitro. The expression of mitogen activated protein kinases (MAPK) and receptor for advanced glycation end products (RAGE) were detected by Western blotting, and the effec...

关 键 词：内皮祖细胞 晚期糖基化终产物 晚期糖基化终产物受体 丝裂原活化蛋白激酶 增殖 凋亡 

分 类 号：R285.5[医药卫生—中药学]