亚麻CAD基因克隆及反义表达载体的构建  

Cloning of Partial Sequences of Cinnamyl Alcohol Dehydrogenase Gene from Flax and Construction of Its Antisense Expression Vector

作　　者：王玉富[1] 黄海燕[1] 薛召东[1] 邱财生[1] 郝冬梅[1] 

机构地区：[1]中国农业科学院麻类研究所,湖南长沙410205

出　　处：《中国麻业科学》2008年第6期289-292,共4页Plant Fiber Sciences in China

基　　金：国家支撑计划;国家行业计划;国家农业部"948"等项目的支持

摘　　要：本试验以亚麻(Linum usitatissimumL.)品种Argos为材料,分离了高纯度的RNA。用简并引物以RT-PCR的方法,克隆了亚麻CAD基因cDNA部分序列,长度为477bp,构建了pGEM-T-CAD质粒。用限制性内切酶EcoRI酶切pGEM-T-CAD质粒和载体pGEM-7Zf(-),进行连接,构建成中间表达载体pGEM-7Zf(-)-CAD质粒。用限制性内切酶XbaI和BamHI双酶切中间表达载体pGEM-7Zf(-)-CAD质粒和pBI121载体,进行连接,构建了亚麻CAD基因反义植物表达载体pBI121-antiNTCAD。The flax(Linum usitatissimum L.) CAD gene was cloned from the variety Argos by RT-PCR using a pair of primers designed from the sequences of existing CAD genes. The cDNA length is 477bp. The plasmid of pGEM-T-CAD was constructed. The pGEM-7Zf (-) vector and the pGEM-T-CAD were cut by EcoR I to release the CAD gene and the pGEM-7Zf(-) fragments, the two fragments were joined together, then a new plasmid pGEM-7Zf (-)-CAD was constructed. The pGEM-7Zf (-)-CAD plasmid and the binary vector pBI121 were cut by Xb...

关 键 词：亚麻 CAD基因 克隆 表达载体 

分 类 号：S563.2[农业科学—作物学]