正交试验优选丹蒲颗粒的醇提取工艺  

Study on Extract Technology of Alcohol in Danpu Keli with Orthogonal Design

作　　者：崔翰明[1] 夏丕芳[1] 程慧平[1] 

机构地区：[1]中国中医科学院广安门医院,北京100053

出　　处：《中国实验方剂学杂志》2008年第10期23-25,共3页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选丹蒲颗粒中丹参和赤芍的醇提取方法及工艺条件。方法:采用正交试验设计考察不同影响因素和水平对芍药苷和丹参酮ⅡA提取率的影响。结果:乙醇回流法芍药苷和丹参酮ⅡA的提取率高于渗漉法和超声法,正交试验筛选出最佳工艺为:8倍量的80%乙醇,回流提取1.5 h,共提取3次。经3批试验验证,对芍药苷和丹参酮ⅡA的提取率分别达到91.2%±4.6%,89.6%±5.2%。结论:优选的醇提工艺简单、高效,对芍药苷和丹参酮ⅡA的提取率高。Objective:To optimize the extract technology of Danshen and Chishao in DanPu Keli with orthogonal design.Method:The different factors for the extracting rate of paeoniflorin and tanshinone Ⅱ_A were researched with orthogonal design.Result:The refluence method is best one in all methods.The major influencing factors were the followings alcohol quantity,alcohol concentration,extraction time and extraction number.The best extracting technology was that adding eight times alcohol(80%),to reflux 1.5 hour and tot...

关 键 词：丹蒲颗粒 芍药苷 丹参酮Ⅱ_A 正交试验 

分 类 号：TQ461[化学工程—制药化工]