反左金丸水提物对人胃癌细胞SGC-7901凋亡及bcl-2、bax基因表达的影响  

Effect of Retro-Zuojin Pill on Apoptosis and Bcl-2,Bax Gene Expression in SGC-7901 Cells

作　　者：余惠旻[1] 彭求贤[1,2] 刘塔斯[2] 杨大坚[1] 陈新滋[1] 

机构地区：[1]普尔药物科技开发(深圳)有限公司,广东深圳518057 [2]湖南中医药大学药学院,湖南长沙410007

出　　处：《中国实验方剂学杂志》2008年第11期28-31,48,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：973计划前期研究专项(2006CB708516)

摘　　要：目的:观察反左金丸水提物(water extracts of Retro-zuojin Pill,WERZP)对人胃癌细胞(SGC-7901)生长的抑制和凋亡诱导效果,及其对bcl-2和bax表达的影响。方法:采用MTT比色法观察不同浓度的WERZP对SGC-7901的生长抑制情况;流式细胞仪检测细胞凋亡率;Hoechst 33258染色观察细胞形态学变化以及Western blot方法检测WERZP对人胃癌细胞SGC-7901中bcl-2、bax蛋白表达的影响。结果:WERZP作用SGC-7901细胞24 h后,细胞存活率显著降低(P<0.05),且与时间、剂量呈依赖关系。流式细胞仪PI单染亚二倍体峰分析,0.1,0.5,1,5和10 mg.mL-1WERZP作用SGC-7901细胞24 h,细胞凋亡率分别为(4.02±1.33),(36.10±0.23),(55.91±0.95),(56.50±1.55)和(60.27±3.58)%,其中(0.5~10)mg.mL-1组与对照组(2.66±1.33)%比较差异均有统计学意义(P<0.01)。Hochest染色结果显示细胞出现核固缩状和颗粒状荧光等典型的凋亡...Objective:To investigate the effect of water extracts of Retro-Zuojin Pill(WERZP) on proliferation and apoptosis of human gastric carcinoma cells (SGC-7901),and the relationship between apoptosis and bcl-2,bax expression.Methods: The cell viability was measured by MTT;the apoptotic rate was investigated by FCM and the morphology was observed by Hoechst 33258 staining.Detection of bcl-2,bax gene and protein expression of WERZP-treated gastric cancer cells were carried out with Western blot method.Results: Af...

关 键 词：反左金丸 人胃癌细胞 凋亡 凋亡机制 

分 类 号：R285.5[医药卫生—中药学]