利用重叠延伸PCR技术扩增长片段DNA  

Amplification of long DNA Fragment by Splicing Overlap Extension PCR

作　　者：魏薇[1] 李凡[1] 陈海如[1] 

机构地区：[1]云南农业大学农业生物多样性与病虫害控制教育部重点实验室,云南昆明650201

出　　处：《云南大学学报（自然科学版）》2008年第S1期86-88,93,共4页Journal of Yunnan University(Natural Sciences Edition)

基　　金：国家自然科学基金资助项目(30660101)

摘　　要：重叠延伸PCR技术是一种通过寡聚核苷酸链之间重叠的部分互相搭桥、互为模板,通过多次PCR扩增,从而获得目的DNA基因片段的方法.该技术可以高效、快捷、经济地扩增长片段DNA,在基因功能研究方面显示出良好的应用前景.综述了重叠延伸PCR技术的原理、特点以及利用该技术构建长片段DNA的方法和注意事项,并对该技术的应用前景进行了展望.The gene splicing by overlap extension PCR(SOE-PCR) is an approach for recombining DNA molecule by precise junctions irrespective of nucleotide sequences at the recombination site.The overlap primers are designed so that the ends of the products contain complementary sequences.When the two products are mixed,denatured,and annealed,the strands have the matching sequences at their 3′ends overlap and act as primers for each other.This techniquel has great advantages in studying on the function of gene.It was s...

关 键 词：重叠延伸PCR(SOEPCR) 长片段DNA 基因 

分 类 号：Q78[生物学—分子生物学]