构建具有神经支配的工程化心肌组织的初步研究  

Preliminary Investigation on Reconstruction of Innervated Tissue-engineered Cardiac Sheet in Vitro

作　　者：王静[1] 郭立国[1] 冯金升[1] 任红茹[1] 

机构地区：[1]中国航天员科研训练中心,北京100094

出　　处：《生物医学工程研究》2008年第4期258-262,共5页Journal Of Biomedical Engineering Research

基　　金：国家自然科学基金资助项目(30530220)。

摘　　要：探索神经生长因子诱导的交感神经元样PC12细胞作为组织工程化心肌组织神经支配研究模型的可行性。用含0.04%EDTA的0.25%胰酶分离新生大鼠原代心肌细胞,然后与NGF诱导的交感神经元样PC12细胞在液态的Ι型胶原中共培养,通过光学显微镜观察、常规H.E.染色、免疫组织化学染色和透射电镜观察等对其进行评价。在三维共培养模型中,NGF诱导的交感神经元样PC12细胞长出神经突起,突起及其上的膨体能够到达跳动的心肌细胞表面,神经突起随心肌细胞一起跳动。说明采用神经元样PC12细胞作为组织工程化心肌神经支配的模型是可行的,神经细胞与心肌细胞可能有支配关系。To investigate the possibility of neuronal PC12 cells being used as models to investigate the innervation of tissue-engineered cardiac tissue.Neonatal rat primary cardiacmyocytes isolated with using 0.25% trypsin containing 0.04%EDTA were co-cultured with the NGF induced sympathetic neuron-like PC12 cells in liquid type I collagen liquid.After that,H.E.staining,immunhistochemistry staining and transmission electron microscopy were used to evaluate it.Result showed that in the 3-D co-culture model,NGF induce...

关 键 词：组织工程 心肌细胞 PC12细胞 共培养 神经支配 神经生长因子 

分 类 号：R318.1[医药卫生—生物医学工程]