新型羟基磷灰石基支架材料的细胞相容性  

Cellular compatibility of the new hydroxyapatite-based scaffold materials

作　　者：王静[1] 孟祥才[1] 李慕勤[1] 赵丽[1] 

机构地区：[1]佳木斯大学省生物材料重点实验室,佳木斯154007

出　　处：《中国体视学与图像分析》2008年第3期204-208,共5页Chinese Journal of Stereology and Image Analysis

基　　金：黑龙江省自然科学基金(No.E2007-28);黑龙江省教育厅骨干教师科研项目(No.1152G043);佳木斯大学科技处科研项目(L2007-81)

摘　　要：目的观察成骨细胞与羟基磷灰石基支架材料的细胞相容性,并探讨中药作为骨支架材料的可行性,为骨组织工程学的临床应用提供依据。方法采用来自胎鼠颅盖骨成骨细胞为种子细胞,冷冻干燥技术制备羟基磷灰石(HA)/壳聚糖(CS)—丝素(SF)—中药淫羊藿(EP)多元混构支架材料,并以HA/CS、HA/CS-SF作为对照进行体外复合培养,通过倒置显微镜、MTT比色法、ALP活性检测和扫描电镜观察分析细胞在材料上的增殖、分化、生长情况。结果成骨细胞在3种材料上皆有细胞附着,3种材料对细胞活性的影响规律为HA/CS-SF-EP优于HA/CS-SF优于HA/CS。结论HA/CS-SF-EP材料有望成为具有发展潜力的新型骨组织工程支架材料。Objective To study the compatibility and growth of osteoblasts cultured with hydroxyapatite (HA)-based scaffold materials and explore the feasibility of epimedium (EP) as functional scaffold materials. Method Osteoblasts obtained from the rat skull were co-cultured in vitro with HA/CS, HA/CS-SF, HA/CS-SF-EP scaffolds prepared by freeze-drying technique. The cell growth and attachment were observed by invert microscopy and scanning electron microscope (SEM). In addition, MTT assay and alkaline phosphatase (A...

关 键 词：生物材料 骨组织工程 复合支架 细胞相容性 羟基磷灰石 

分 类 号：R318.08[医药卫生—生物医学工程]