Fas配体基因对大鼠颈动脉损伤后血管中层平滑肌细胞密度的影响  

Effects of adenoviral vector mediated Fas ligand gene introduction on the medial layers of vascular smooth muscle cell density after rat carotid artery injury

作　　者：陶红[1] 陈立新[2] 岑敏[1] 林江[1] 

机构地区：[1]暨南大学第二临床医学院深圳市人民医院体检中心 [2]暨南大学第二临床医学院深圳市人民医院超声科

出　　处：《中国临床药理学与治疗学》2008年第12期1401-1403,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨腺病毒载体介导Fas配体(FasL)基因导入对大鼠颈动脉损伤后血管中层平滑肌细胞密度的影响。方法:实验分治疗组(Ad-FasL组)和正常对照组两组(Ad-βgal组),每组动物均为6只。利用重组腺病毒载体分别将Ad-FasL、Ad-βgal导入大鼠颈动脉球囊损伤的内膜,3、14d后观察其对血管中层平滑肌细胞密度的影响。结果:与正常对照组相比较,通过腺病毒载体介导导入FasL基因的被球囊损伤的大鼠颈动脉,3d后损伤血管中层平滑肌细胞密度降低,14d后血管中层平滑肌细胞密度恢复到正常水平。结论:FasL可抑制血管中层平滑肌细胞密度,从而抑制球囊损伤后的内膜增生。AIM:To investigate the effects of Fas ligand(FasL)gene transduction mediated by adenoviral vector on the density of medial layer vascular smooth muscle cell after rat carotid artery injury.METHODS:SD rats were divided into treatment group(Ad-FasL group)and normal control group(Ad-βgal group)(n=6).Ad-βgal,Ad-FasL gene were transduced into endomembrane of balloon injury of rat carotid artery using recombination adenovirus vector,the density of medial layer vascular smooth muscle cell was observed after 3,14 d...

关 键 词：FAS配体 血管中层平滑肌细胞 腺病毒 基因疗法 

分 类 号：R543[医药卫生—心血管疾病]