环孢素A改善异丙肾上腺素所致心肌损害和纤维化可能由抑制内皮素通路所介导  

Protective effects of cyclosporine A on myocardial damage and fibrosis induced by isoproterenol via ET pathway

作　　者：彭洪军[1] 季晖[1] 戴德哉[1] 戴茵[1] 

机构地区：[1]中国药科大学药理研究室

出　　处：《中国临床药理学与治疗学》2008年第9期966-971,共6页Chinese Journal of Clinical Pharmacology and Therapeutics

基　　金：国家自然科学基金项目(30670760)

摘　　要：目的:研究环孢素A(Cyclosporine A,CsA)对异丙肾上腺素(isoproterenol,Iso)诱导心肌损害和纤维化的改善,探讨内皮素通路在其过程中的作用。方法:SD大鼠给予Iso(2 mg·kg-1·d-1,s.c.)10 d,建立大鼠心肌损害和纤维化病变。CsA治疗组预先给予CsA(25 mg/kg,s.c.)一次。结果:Iso损害心肌,引起心功能下降,使左室心肌中羟脯氨酸(hydroxyproline,Hyp)含量增高,内皮素-1(ET-1)及受体(ETAR)、结缔组织生长因子(CT-GF)和基质金属蛋白酶-9(MMP-9)mRNA和蛋白表达上调。CsA明显改善心功能,降低Hyp含量,减轻升高的mRNA和蛋白表达。结论:CsA改善Iso引起的大鼠心功能损害和纤维化,与抑制内皮素通路相关。AIM: To investigate the protective effects of Cyclosporine A(CsA) on myocardial damage and fibrosis induced by isoproterenol(Iso) in rats and the role of ET pathway.METHODS: Myocardial damage and fibrosis in rats were produced by isoproterenol(2 mg·kg-1·d-1,s.c.) for 10 days.In CsA-treated group,the rats were pretreated by CsA(25 mg/kg,s.c.).All the animals were subjected to cannulation through left carotid artery to measure LVSP,LVEDP,and ±dp/dtmax under urethane anesthesia.The contents of hydroxyproline(H...

关 键 词：环孢素A 内皮素-1 内皮素受体A 结缔组织生长因子 基质金属蛋白酶-9 

分 类 号：R965.2[医药卫生—药理学]