缬沙坦对自发性高血压大鼠肾脏核因子-κB蛋白表达的影响  

Effects of valsartan on nuclear factor-κB protein expression in the kidney of spontaneously hypertensive rats

作　　者：杨玉雯[1] 王德国[1] 孔祥权[1] 徐承华[1] 

机构地区：[1]皖南医学院附属弋矶山医院心血管内科

出　　处：《中国临床药理学与治疗学》2008年第9期1002-1004,共3页Chinese Journal of Clinical Pharmacology and Therapeutics

摘　　要：目的:探讨自发性高血压大鼠(SHR)肾组织NF-κB原位表达变化以及AT1受体阻断剂缬沙坦对其的影响。方法:16只SHR随机分成SHR组和SHR加缬沙坦药物干预组(1 mg.kg-1.d-1),另8只WKY大鼠为正常对照组。分别在实验第2、4、6、8周末测定大鼠尾动脉收缩压(SBP);实验结束取各组大鼠血浆测定血浆中肾素、血管紧张素II(AngII)水平;取出各组大鼠两侧肾脏组织,分别运用免疫组化原位测定肾组织中NF-κB蛋白表达,并进行半定量分析。结果:SHR组SBP和血浆中肾素活性以及AngII水平与WKY大鼠相比显著增高,缬沙坦治疗组SBP显著降低,但血浆中肾素活性以及AngII水平明显高于SHR组和WKY组。在蛋白水平,WKY组大鼠肾组织中NF-κB表达较低,SHR组肾小球和肾小管中NF-κB高度表达;使用缬沙坦干预后,NF-κB表达显著降低。结论:在蛋白水平对NF-κB表达的调控可能参与缬沙坦的降血压效应和肾保护作用。AIM: To observe the local expression of nuclear factor-κB(NF-κB) in spontaneously hypertensive rat(SHR) kidney and effects of AT1 receptor contagonist valsartan.METHODS: 16 SHRs were randomly divided into two groups: SHR control group and valsartan group.Another 8 WKY rats act as normal control group.Systolic blood pressure(SBP) of SHR was measured at the beginning and the end of 2,4,6 and 8 weeks of intervention treatment.Radioimmunoassay was used to determine the activities of rennin and angiotensin II(An...

关 键 词：自发性高血压大鼠 核因子-ΚB 缬沙坦 

分 类 号：R544.1[医药卫生—心血管疾病]