女性生殖道内乳杆菌菌种分类学研究  

Taxonomy study on Lactobacillus of female genital tract

作　　者：何亮[1] 曾忠铭 陈群[3] 

机构地区：[1]深圳市慢性病防治院病原检验科,广东深圳518020 [2]深圳市南山医院,广东深圳518020 [3]广东医学院微生物学教研室,广东广州524023

出　　处：《中国医疗前沿》2008年第15期1-2,5,共3页China Healthcare Innovation

基　　金：国家"十五"攻关课题(2004BA720A05-02)

摘　　要：目的观察女性生殖道内乳杆菌属各种乳杆菌在阴道中的分布差异。方法采用Nugent评分标准对阴道分泌物进行镜检,接种乳杆菌MRS培养基分离培养,使用API50CHL鉴定条码对乳杆菌进行分类鉴定。结果(1)有39例受检者经Nugent评分及微生态学检查诊断为正常,有2例诊断为细菌性阴道病。(2)共鉴定出9个种41株乳杆菌,其中有2株只在厌氧环境中生长,其余均在厌氧及CO2环境中生长。(3)鉴定菌种包括:嗜酸乳杆菌16株、类干酪乳杆菌9株、卷曲乳杆菌7株、德氏乳杆菌4株、发酵乳杆菌2株、短乳杆菌2株、植物乳杆菌1株、林氏乳杆菌1株、乳杆菌霍氏亚种1株。结论乳杆菌是阴道内的正常菌群,,不同个体生殖道内乳杆菌菌种不尽相同。Objective To investigate the distribution of Lactobacillus in the vagina of female genital tract. Methods Nugent standard was used to diagnose the vaginal discharge , MRS agar was used to culture Lactobacillus and API 50 CHL was used to identify Lactobacillus . Result 39 women were diagnosed as the normal and 2 patients were diagnosed as the Bacteria Vaginosis patients; 9 species 41 strains Lactobacillus were detected, and two of them grew in anaerobic environment only; Lactobacillus acidopgilus 1(12), Lact...

关 键 词：阴道 正常菌群 乳杆菌 API鉴定条码 分类 

分 类 号：R711.3[医药卫生—妇产科学]