丙酮酸乙酯对脓毒症小鼠相关脏器的保护作用  

Protective effect of ethyl pyruvate on the related organs in septic mice

作　　者：遆冬冬[1] 邓子辉[1] 薛辉[1] 张凯[1] 林季[1] 颜光涛[1] 

机构地区：[1]解放军总医院基础医学研究所生化研究室,北京100853

出　　处：《感染．炎症．修复》2008年第4期217-220,共4页Infection Inflammation Repair

基　　金：国家自然科学基金面上项目(30670821)

摘　　要：目的:探讨丙酮酸乙酯(EP)对脓毒症小鼠肾脏、肝脏、肺脏组织的保护作用。方法:采用盲肠结扎穿孔术制备小鼠脓毒症模型。100只雄性昆明小鼠随机分为假手术组、脓毒症组、EP预保护组、EP保护组,后三组又设立1h、3h、6h组,每组10只。用分光光度法检测血清碱性磷酸酶(ALP)活性,肝脏、肺脏组织匀浆液中总抗氧化能力(TAOC)和丙二醛(MDA)含量。结果:EP预保护组6h、EP保护组6h血清ALP较损伤组6h显著降低。EP预保护组3h、6h及EP保护组3h、6h肝脏、肺脏组织总抗氧化能力较损伤组相应时间点明显升高。EP干预后,受损肝脏、肺脏组织丙二醛无明显变化。结论:EP可能对脓毒症后。肾脏、肝脏、肺脏等受损脏器起一定的保护作用,其机制与影响氧自由基有关。Objective:To explore the protective effect of ethyl pyruvate(EP)on liver,lung and kidney in mice with sepsis.Methods:Sepsis model was reproduced in mice by cecum ligation and puncture.One hundred male Kunming mice were randomly divided into sham-operation group,sepsis group,EP-pretreatment group and EP- treatment group,and the latter three groups were further divided into 1,3 and 6 hours subgroups,each of them consisted of 10 mice.Serum alkaline phosphatase(ALP),and two enzymes related to synthesis of oxyge...

关 键 词：丙酮酸乙酯 脓毒症 氧自由基 

分 类 号：R459.7[医药卫生—急诊医学]