HPLC同时测定大鼠肠循环液中盐酸小檗碱和酚红的浓度  

Simultaneous determination of berberine and phenolsulfonphthalein in the solution of in situ intestinal loop by HPLC

作　　者：胡慧玲[1] 王战国[2] 刘芳[2] 杜青青[2] 何瑶[1] 

机构地区：[1]成都中医药大学,四川成都6100752 [2]四川大学华西药学院,四川成都610041

出　　处：《成都中医药大学学报》2008年第4期67-69,共3页Journal of Chengdu University of Traditional Chinese Medicine

基　　金：成都中医药大学科研基金项目(编号:ZKyB2007031)

摘　　要：目的:同时测定大鼠肠循环液中盐酸小檗碱和酚红的浓度。方法:采用RP-HPLC法,色谱柱为Welchrom-C18(5μm,250 mm×4.6 mm),流动相为乙腈-水-磷酸-三乙胺(30∶70∶0.2∶0.1),柱温30℃,流速1.0 mL/min,检测波长270 nm。结果:方法精密度及线性关系良好,盐酸小檗碱和酚红低、中、高浓度的回收率分别为99.66%(RSD=0.49%)、99.26%(RSD=0.68%)、99.69%(RSD=0.62%);99.85%(RSD=0.70%)、99.44%(RSD=0.86%)、99.72%(RSD=1.32%)。结论:该法操作简便,结果准确,灵敏度高。Objective:To determine the concentrations of berberine and phenolsulfonphthalein in the solution of in situ intestinal loop simultaneously.Method:An RP-HPLC method was established with a Welchrom-C18(5 μm,250 mm×4.6 mm).The mobile phase was acetonitrile-water-phosphoric acid-triethylamine(30:70:0.2:0.1) and the column temperature was set at 30 ℃.The flow rate was 1.0 mL/min with detective wavelength of 270 nm.Results:The precision and the linear relation of this method were acceptable.The recoveries of berb...

关 键 词：盐酸小檗碱 酚红 在体肠循环 高效液相色谱法 

分 类 号：R285[医药卫生—中药学]